I’m working on a Philosophy case study and need a sample draft to help me study.
A brief proposal on the topic of your research paper (see below) is required. You must tell
me what societies you have chosen, list your references, and explain why you choose those
groups. Your paper will consist of a comparison of two non-Industrial societies (e.g., two 4
World groups; we will discuss this process at some length in class). You are required to find two
“Case Studies in Cultural Anthropology” from the library and compare and contract them
following the general outline provided below. If you do not find a good case study in the USD
library, search the SDSU library catalog and order what you want from the “circuit” (ask a
librarian if you need to). It is possible to find two such societies using the eHARF files in the
library (see below). Topics that I chose are ( Cherokee and Creek ) cultures. The proposal paper shouldn’t be more then 2 to 3 pages.For the eHARF, you should use this link:
https://sandiego.idm.oclc.org/login?url=http://ehr…The link is only preffered to get the paper from.
San Diego State University Cherokees and Creek Cultures Discussion
Competency Evaluate the importance of personal and professional lifelong learning, including the interrelationships of clinical reasoning and clinical judgment. Scenario As a BSN prepared Registered Nurse, you are due to renew your license for the first time. You and your manager overhear other nurses complaining about the continuing education requirement. Your manager suggests you create a handout for the staff lounge bulletin board regarding the importance of lifelong learning as it pertains to clinical reasoning and judgment. To help you get started, she suggests you read the Institute of Medicine (IOM) report titled The Future of Nursing: Leading Change, Advancing Health (2010) so that you will understand “why” it is so important. Instructions Read Section 4 of the IOM report (2010) titled Transforming Education and respond to the following questions in a Word document. https://www.nap.edu/read/12956/chapter/9#164 Describe an initiative that resulted from the IOM report. Discuss the impact of the identified initiative. Summarize the interrelationships that exist between lifelong learning, clinical reasoning, and clinical judgment.Provide clearly stated detailed description of an initiative that resulted from the IOM report that includes a national, state or local initiative.Provide clearly stated, detailed summary of the impact of the identified initiative, including the impact of nursing, nursing education, healthcare facilities, and client care.Provide clearly stated, detailed summary of the interrelationships that exist between lifelong learning, clinical reasoning, and clinical judgment.Do not use 1st person in scholarly writing. Use 3rd person only. APA format. Formal style reflected throughout the document, including no spelling, grammar, or APA format errors present. Adequate references with adequate in text citation. Please do this assignment using only research published within the past 5 years. Plagiarism free
Remington College Tampa Campus ?Lifelong Learning is Part of Nursing Practice Paper
Hydraulics Lab report
Hydraulics Lab report.
I’m working on a engineering writing question and need support to help me understand better.
I’m working on an engineering report and need support to help me study.I have a lab report for water resources engineering and I did the excel calculations I just need help writing the reportIntroduction (10 points)The introduction should be a paragraph that contains the goals of the lab and an overview of what the reader can expect to find within the report. State the objective of the lab exercise. Though this is provided in the lab documents, the purpose should be restated in your own words. Provide a brief overview or “roadmap” of the report contents.Results: This is the only section in which results should be introduced, present your final results from the analysis(tables). Start this section with a brief description of what your data shows. Give your reader a context in which to view the data. Present only your information (raw data, tables from excel sheet, graphs…etc.)Discussion: You should explain your results and their ramifications by answering the questions provided in the procedures (usually it is 3 to 5 questions). You can include details of the principles, relationships, generalizations, and consequences of your experiment. You must demonstrate that you understand your investigation and corresponding results. Be sure to relate your interpretation and discussion back to your objectives. Do your data answer your original question? You should also include a discussion of possible errors and exceptions. If relevant or available, discuss how your results compare pertinent standards.A good conclusion is usually quite short. A few sentences should be sufficient. Restate your numerical results, but do not editorialize about your results.Conclusion: Summarize the major points of your memo. A good conclusion is usually quite short. A few sentences should be sufficient. Restate your numerical results, but do not editorialize about your results..References (2.5 points)List all your reference writing HintsA lab report is considered a technical report. As such, it must be professional and neat. The report must be written with a word processor of your choice. All drawings must be made with a straight-edge and clearly labeled or drawn on the computer. Plots are an integral part of technical reports and must be professional:•Plots must be computer generated;•Scales must be readable to the same accuracy as the data obtained duringthe test;•Axes must be labelled;•Units of variables must be shown;•Various run results must be distinguished by different symbols and/or colors and each curve must be identified by a legend or a title;•Curves derived from data must show experimental data points;•Graphs must be drawn as smooth curves that represent an average of the experimentally determined data.•Curves derived from an equation should contain no symbols, but show the equation of the curve;•Graphs must be labeled/legendA portion of the grade for each lab memo will be based on the writing itself.•Be as clear, direct and concise as you can. Grammar, spelling, clarity, and style will all be considered. •Pay attention to tense. When describing established, factual material, the present tense can be used. A discussion section is generally written in the present tense. Even though you are discussing past results, you are interpreting them now. Completed procedures are usually referred to in the past tense.
Hydraulics Lab report
Strategic Business Plan
online homework help Strategic Business Plan. Developing a Strategic Business Plan Either in the start up process or when re-inventing one’s business development, the design of a strategic business plan is an indispensable step towards a successful and viable business. Strategic planning involves setting up a sound and multifaceted plan or strategy to follow over a defined time period.It can involve all aspects of the business, or just a small part of it i.e. a selected department such as the marketing department.However, this does not mean that strategic business planning is only for large scale businesses since it can also benefit the small business, especially at start up, when the business sets its first goals and establishes itself in the business landscape. Writing up a business plan is an important step of a starting business, since most lending bodies will not authorize loans in absense of a detailed business plan.Why is it important? Imagine sailing off for a voyage in the sea and not planning for resources or supplies that you might need. Developing a business plan is the only way to determine the allocation needs, personnel, utilities, marketing goals, outreach and all valuable aspects that make your business useful and unique in the sea of business. However, strategic planning is a process employed in any time period in a formal business administration, since it usually comprises of a long range planning process of a specific or broader managerial aspect, that will in a time-frame manner set the outline of the business’ goals and image and ultimately form its policy. To be successful, a strategic business plan, either at start up or as a means of sustainment of a particular policy, should provide with day to day reference for the decision making in organization and management of the business and should provide a template against which all decisions can be evaluated (Goodstein et al ,1993).Ackoff’s (1981) typology of planning, provides with several points to the understanding of the planning process, identifies as a key step in the business or organization’s decision to adhere to the long term goals of strategic planning.These are l Reactive planning ( planning through the rear view mirror) l Inactive (going with the flow) l Preactive or future planning l Proactive or designing the future planning. The most challenging and demanding type of planning is the proactive planning, which is based on the beleif that the organization or business itself, is responsible for shaping its future and thus can do differently i.e. to solve an anticipated problem.In order for an administration to sustain an applied strategic planning process in its midsts, it is anticipated that efforts are made to develop an openness for new ideas and allow for envisioning in serving the future. An area where envisioning is particularly useful is the mision development : who the business customers are, how will the business go about its tasks and the business raison d’etre. Moreover, the strategic planning process must be strongly intercalated with the organizational culture of the business, the latter being the social context in and through which the business performs its work. This connection will facilitate the easy transmission to the workers and administrators of the mission of the business, the specific operational goals and the means to acheive them. After the decision and contexts of the strategic planning are made, several steps are indispensable in the successful inplementation of the process: 1st setting the stage for planning: understanding the way planning works, the values scan and mission formulation 2nd Setting strategic business directions : strategic business modeling such as performance auditing, gap analysis 3rd Implementing the strategic plan : integrating action plans, contigency planning and implementation. In conclusion, a strategic business planning process is an expansion of the traditional business plan method towards the future developmentof the business, that formulates a strategy in anticipating change and maintaining its status in the business arena. Several steps are necessary in implementing a viable and successful strategy plan, most of which require serious documentation, market research and official design and control. In several examples in history, an inspired and well grounded strategy plan has been the means to ground-breaking enterpreneurships and immense success. REFERENCES: Ackoff (1981) Creating the corporate future. New York : John Riley Goodstein LD, Nolan T, Pfeiffer JW (1993) Applied strategic planning. Mc Graw Hill Strategic Business Plan
Importance of Cell Cultures
Importance of Cell Cultures. Introduction Cell culture is an extremely widely used process by which cells are removed from their natural environment and grown artificially under controlled and monitored conditions. It occurs in vitro, or in glass, more specifically in multicellular eukaryotic cells. The cells may be removed from their habitat directly and disaggregated with enzymes or mechanically before harvesting, or they may be a derivative of a cell line that has been created previously. It was adapted from a practice used in the early 1900’s and since then it has expanded and advanced research and scientific knowledge enormously. The conditions required for each culture vary, however the artificial environments conditions are consistent. It must consist of a suitable vessel which contains a medium that provides vital nutrients such as amino acids, vitamins, carbohydrates and minerals. Growth factors and hormones are also needed, as well as oxygen and carbon dioxide. It must monitor and regulate physico-chemical environment which includes pH and osmotic pressure, as well as temperature. Temperature is kept at 37°C, CO2 levels at 5% and humidity at 95%. Cell cultures are an extremely important tool for healthcare scientists. They provide a model system for physiology and biochemistry of selected cells to be studied. By examining their physiology their aging pathway can be studied and their biochemistry allows processes such as metabolic rate to be observed. The cells interaction with drugs could also be observed which proves a useful tool for drug screening programs, clinical trials and pharmaceutical companies. Whatever the purpose for using cell cultures, it is an extremely consistent and reliable process that has good reproducibility of results that can be obtained using a batch of clonal cells. Primary cell cultures are cultures that grow and maintain cells dissociated from their parental tissue via mechanical or enzymatic methods. They can be either adherent or suspension cells. Adherent cells are also known as anchorage dependent cells because they require attachment for growth. These cells are usually derived from organs such as the kidney where they are immobile and implanted into connective tissue. Suspension cells are the opposite and don’t require attachment to the culture vessel for growth. These types of cells are anchorage independent cells. They are cells that derive from the blood, where they aren’t attached to anything but are still suspended e.g. in plasma like lymphocytes. A secondary culture is a primary culture that has been sub-cultured. The sub-culture (passage) occurs when the cells are transferred from a culture vessel to another. This provides fresh nutrients and space for continued growth, because a primary culture has a finite life span. Common primary and secondary lines can be found in Table 1. After the first sub-culture, the culture becomes known as the cell line. Cells only undergo a finite number of replication cycles before cell death. This means that some cell lines will be finite cell lines. However, some cells undergo transformation. This can occur spontaneously but can also be virally induced in vitro. Undergoing transformation gives the cell the ability to divide infinitely, such as HeLa cells. The HeLa line is the oldest and most commonly used continuous cell line. Cervical cancer cells biopsied from Henrietta Lacks in 1951 show that they are remarkably durable and prolific. In 2012, Turner published a paper documenting its importance in the development of the polio vaccine. Table 1: Summary comparison table of cell line examples, their uses and origins Cell Line Original Cells Example paper Henrietta Lack (HeLa) cell line Cervical cancer cells from a biopsy from Henrietta Lacks, first immortalised cell line (Turner, 2012) COS-7 cell line Fibroblast-like cells from African Green Monkey kidney tissue (Vacante et al., 1989) SH-SY5Y cell line Neuroblastoma cells from a biopsy of a 4-year old female TO FIND AND ENTER!!! Hep G2 cell line Hepatocellular carcinoma cells from a biopsy of a 15-year old male’s liver (Mersch-Sundermann et al., 2004) Jurkat cell line T-lymphocyte cells in the blood of a 14-year old male leukaemia patient (Wang et al., 2012) The COS-7 cell line is a line derived from African green monkey kidney tissue. It is used in research against SV40, a cancer causing virus that was hidden in the polio vaccine (Vacante et al., 1989). The Hep G2 cell line is another continuous cell line of hepatocellular carcinoma. It plays a vital part in the research of human liver diseases by being a model for intracellular trafficking (Mersch-Sundermann et al., 2004). Jurkat cells, another continuous line, are a line of lymphocyte cells used to study leukaemia, T-cell signalling and HIV (Wang et al., 2012). This review will explore the use of cell lines in the laboratory and their applications. SH-SY5Y will be a particular focus, and will explore the application and importance of the cell line as one of the only lines used to study neuronal function and differentiation. SH-SY5Y cell line SH-SY5Y cells are a derivative cell line used majorly in scientific research. SH-SY5Y originally was cloned from a biopsy of bone marrow derived line called SK-N-SH, and then named as SH-SY. The biopsy was from a 4-year old female with neuroblastoma. This was subcloned again to make SH-SY5 and subcloned once more to form SH-SY5Y. Because this cell line has been derived from a primary source, it is a secondary culture. There is new, fresh growth medium in which the cells are suspended not attached, making them anchorage-independent cells in the cell line. They have been widely used since the 1980’s, due to their ability to express dopaminergic markers and neuronal function such as neurodegenerative processes. Because of these characteristics, they play a major role in the research of Parkinson’s disease. As mentioned before, the cells are subcloned. This process of sub-culturing is also known as cell passaging. Cell passaging is where a new microbiological culture is created by transferring a sample, or all, of a cell culture to a different growth medium. This process prolongs the life of the organism, renews depleted nutrient levels and also increases the concentration of cells in the culture. Cells cannot be held in their primary culture indefinitely because continual cell activity means there will be a gradual rise in toxic metabolites. For SH-SY5Y cells, there is a recommended limit of cell passaging. Passage numbers can affect cell physiology and morphology, protein expression and transfection efficiency, so the limit has been set to 20 to prevent unreliable and irreproducible results being collected. Use of SH-SY5Y cell line in research As conferred, SH-SY5Y is one of the only cell lines that can be used as a model system for neuronal function investigation. It is particularly good for investigating the effective of oxidative stress on neuronal cell lysis. Reactive oxygen species (ROS) at specific concentration are essential for standard cell function however over exposure to ROS is harmful to cells. There are 2 globin’s whose functions are still unclear. Neuroglobins (NGBs) and cytoglobins (CYGB) role has been suggested to involve detoxifying the effects of over exposure. Excessive ROS has been known to cause cell lysis after ischaemic strokes. By investigating the correct levels and limit levels of ROS it can have an enormous clinical impact on stroke recovery and treatment. Forde et al. investigates the effect of NGB and CYGB on the detoxification of ROS. The influence of cell lysis of surplus ROS is the primary focus, more explicitly hydrogen peroxide. SH-SY5Y cells were cultured at a ratio of 1:1 of Dulbecco’s minimum essential medium (DMEM) and Ham’s F-12 nutrient medium along with 10% foetal bovine serum at 5% CO2 atmosphere. The culture was maintained at 37°C in a humidified 95% atmosphere. L-glutamine provided an energy source and sodium bicarbonate acts as a pH buffer. Growth factors and non-essential amino acids (NEAA) are also present and standard factors. In the culture, penicillin and streptomycin are the selected antibiotics used. The pathogen cell membranes are broken down to prevent infection. In cell lines cross contamination can be rife, so using antibiotics prevents this and induced recombinant protein expression. Apart from preventing the obvious infection risk, if there is contamination there will be unreliable and inaccurate results. However, antibiotic resistance means that there may always be a level of low contamination. The prolonged use means antibiotics are only used where absolutely necessary so that it prevents these problems, such as in initial cell lines to prevent contaminated cells being carried on in sub cultures and protecting stock solutions. Methods and Materials After SH-SY5Y had been cultured, they then were transfected. NGB and CYGB plasmids were transfected with SH-SY5Y by nucleofectin. Nucelofectin is a transfection method that requires the use of electrode force to administer specific voltage. Reagents and electrodes produce the conditions required for transfection, which increases the permeability of the target cell. This allows the genetic material present in the culture to transfect into the globin plasmids. This is a reliable mechanism and produces good rates of success. After transfection, the globins were fused with the GFP gene by PCR-amplification. The NGB to CYGB region was amplified and digested with restriction enzymes. Ligation was then performed directly after in to PEGFP-N1 vectors. The culture cells were briefly re-suspended in nucelofactor solution and nueclofected with 2μg of plasmid DNA, producing a final result of NgbN1-pEGFP and CygbN1-pEGFP fusion proteins. These produce a yield of 40% eFP positive cells. The PCR identified the expression To examine the success of the transformation, PCR determined the expression of the globins. PCR measures the expression by recording the amount of mRNA present before and after amplification. For reactions involving GFP, fluorophene is added to act as a marker and signal upon excitation. Upon examination, over a 12 hour period there was upregulation 12 hours after transfection, meaning the globins were transfected successfully. This examination isn’t thorough enough to provide evidence of success. A western blot was performed to ensure thorough examination. Protein expression can be detected by electrophoresing the proteins through a 10% polyacrimide gel. The proteins were transferred on to a western blot by being electroblotted to an Immobilon P membrane. After staining with primary polyclonal antibodies they were incubated with a secondary antibody, and probed for antibodies upon completion with Supersignal West Pico Chemiliminescent substrate. Figure 1 displays the result of the western blot. Importance of Cell Cultures
Moraine Valley Community College Ancient Times Work Influence in Modern World Essay
Moraine Valley Community College Ancient Times Work Influence in Modern World Essay.
Im giving you plenty of time to work on it. NO PLAGIARISM. TOPIC: Students will have to find the most important connections that we find
in the present day with the ancient times and the influence of the ancients in our lives: some examples of topics to
cover are words and idiomatic expressions, traditions, technology of any kind, ideas, beliefs, politics, etc. It can be
done in organized sections by groups of civilizations (Egyptians, Sumerians, Babylonians, Greeks, Romans,
Chinese, etc.) or by a list of different topics (vocabulary and expressions from the Greeks, Romans, etc.; technology,
warfare tactics, traditions, construction techniques, religion, philosophical thoughts, etc.). In other words, you will
have to provide a list of main contributions, influences and connections of the ancient civilizations covered in this
class with the present world, and those most relevant for the process of globalization and common good, or
general progress. Each annotation of the list will have a short paragraph describing, explaining, and evaluating the
importance of that contribution, influence or connection. Typed double-spaced; Font: Times New Roman; Size: 12.
Bullet point paragraphs accepted as far as they are well explained. No limit on its extension, but a minimum of 8
pages is required, and the use of at least three (3) different sources. List of sources/bibliography required at the
end of the paper.
5. MANDATORY DOCUMENTARIES –MUST WATCH
Moraine Valley Community College Ancient Times Work Influence in Modern World Essay