Get help from the best in academic writing.

St Francis College Eliminating COVID19 by Exploring the Solar System Essay

St Francis College Eliminating COVID19 by Exploring the Solar System Essay.

In the last several weeks we have covered a lot of material about the earth and earth sciences. Reading Chapter 17 should provide an expanded appreciation for the uniqueness of the earth’s geology, atmosphere, and chemistry as a life sustaining planet. This chapter provides a brief description of many different objects in our solar system: other planets, their moons, rings, the asteroid belt, etc. Peruse the chapter and choose two destinations that strike you as being particularly interesting for humanity to visit (permanently). Your choices shouldn’t be whimsical; they should respond to a need that humanity has.Earlier in this course, we discussed a few of the needs and challenges that humanity faces going forward (economics, population, energy . . . and there are others not covered in this course). Is it possible that outposts or larger settlements elsewhere in the solar system can help humanity overcome some of these challenges? Using some of the basic sciences we covered initially in this course, we can also consider the challenges to getting to that location, establishing an outpost, and not dying. Sections 19.12 and 19.13 are just a couple of pages that might give you some ideas.Write an essay about your choices (two of them). The essay should identify a challenge to humanity (scientific, economic, cultural, political, or any other), and explain how such an outpost would be beneficial. It should also discuss the major challenges or considerations in establishing such an outpost (can be technological, economic, societal . . .). One paragraph on each destination, plus a few extra sentences to set a good tone or pull things together, should be sufficient.This essay is not a research paper. I am not looking for an exhaustive list of well sourced ideas, although some research is expected. I am looking for your half of a conversation that might occur between you and some random acquaintance spontaneously. One of the goals of the course is to give you the ability to have science conversations on the fly. As you are perusing Chapter 17, some ideas should come to you. Just write them down (coherently, good grammar and style is still important) and submit it here. Put in something of yourself; integrate your own experiences and thoughts on the future of humanity in order to make something that is genuinely you.To set the mood, you could imagine that you are on a road trip and the radio just had a news story about the next comet visiting the earth. To start a conversation, you say “Do you know where I’d like to go in the solar system? Planet H, because one of the problems here on earth is . . . and once we have an outpost there, we could solve it. Of course getting there would be hard. We’d have to solve these problems first, but I think it could be done this way.”Or perhaps you hear about an earthly problem on the radio, and you say “There could be a solution to that problem over there on that asteroid. The problem in using that solution is . . . . To overcome that challenge, we could use . . .”So, first a couple sentences about the problem, destination, and benefits, then a statement of the biggest obstacles, and a couple sentences about possible directions to take to solving the problems. Do that twice. The original post is worth 30 points.
St Francis College Eliminating COVID19 by Exploring the Solar System Essay

LDR 615 Grand Canyon University Kottlers Change Model Research Paper

LDR 615 Grand Canyon University Kottlers Change Model Research Paper.

You will utilize your change model for this assignment. Review the feedback submitted by your instructor on your previous change model assignment. Make any changes or modifications necessary for the submission of this assignment.Evaluate the performance of your organization or department. Identify an area that would significantly benefit from initiating a change. Write a paper (1,500-1,750 words) in which you describe the particular area you propose to address through a change initiative. Include the following for your company:Discuss the issues in this area and the current outcomes as a result of the issues.Describe the external and/or internal driving forces, contributing issues, and the people affected.Evaluate the stakeholders involved and discuss how they will be affected by your change initiative.Clarify your role and responsibility as a change leader. Discuss the leadership theory (or theories) you will use to guide the change process. Discuss the change agents you need to recruit in order to successfully implement your change. Describe the roles of these change agents.Utilize your change model to develop strategies: (a) Explain the relevance of this model to your organization; and (b) Present the strategic aspects using your model. Be sure to clearly define the purpose of each aspect, the people involved, and the actions that need to be taken.Identify, or predict, the potential barriers to change. Discuss possible ways to overcome these obstacles, including methods for dealing with emerging or unforeseen circumstances that could impede implementation.Describe the evaluation methods you will use to determine the level of success of your change initiative. Discuss what metrics or measureable determinates you will use.Propose strategies to anchor change or support continuous change.Establish how your change plan supports the organizational mission/goal, genuinely addresses stakeholder concerns, and will serve as an equitable contribution for the community or society overall.
LDR 615 Grand Canyon University Kottlers Change Model Research Paper

Just because the testing is done and the evidence is reviewed, that does not mean that the audit is complete.

essay writing service free Just because the testing is done and the evidence is reviewed, that does not mean that the audit is complete..

Assignment 1: Completing the AuditJust because the testing is done and the evidence is reviewed, that does not mean that the audit is complete.In this assignment, you will demonstrate your understanding of the necessary steps to complete an audit, as well as determine the appropriate audit opinion to issue.The questions listed below are found in Chapters 16 and 17 of Principles of Auditing. Answer the questions and submit your work to the instructor as outlined below.Checklist:Respond to the following QuestionsChapter 16Question Requiring Analysis: 16-31Question Requiring Analysis: 16-35Chapter 17Question Requiring Analysis: 17-24Objective Question: 17-26Please review the complete assignment details and rubric.Assignment 2: Additional ServicesIn addition to auditing public companies, accountants can provide services for nonpublic companies and services that do not require an annual external audit. You will demonstrate your understanding of these additional services that can be provided.The questions listed below are found in Chapter 19 of Principles of Auditing. Answer the questions and submit your work to the instructor as outlined below.Checklist:Respond to the following questionsChapter 19Question Requiring Analysis: 19-25Question Requiring Analysis: 19-27Objective Question: 19-29Objective Question: 19-33Please review the complete assignment details and rubric.Assignment 3: Services Provided without VerificationProvide an original and substantive response to the questions posed in your assignment in a minimum of 200 words. Please review the complete assignment details and rubric.After reading and discussing how important it is for auditors to gather evidence to back up client claims, you are now introduced to accounting services where it is unnecessary to verify management’s information.In providing these compilations, how comfortable would you be in preparing financial statements based on data that you are not going to verify?What concerns would you have signing off on these compilations as a CPA?Because you are not performing audit services on the compilations, how do you know if the information you are given is true?What types of questions could you ask your client that would fall within the realm of compilations without crossing the line into auditing? Assignment 4: Services for Non-Public CompaniesIn addition to auditing public companies, accountants can provide services for nonpublic companies and services that do not require an annual external audit. You will demonstrate your understanding of these additional services that can be provided.The questions listed below are found in Chapter 20 of Principles of Auditing. Answer the questions and submit your work to the instructor as outlined below.Checklist:Respond to the following questionsQuestion Requiring Analysis: 20-25Question Requiring Analysis: 20-28Objective Question: 20-29Objective Question: 20-31
Just because the testing is done and the evidence is reviewed, that does not mean that the audit is complete.

Binding of QNB and Atropine to Muscarinic Acetylcholine

Binding of QNB and Atropine to Muscarinic Acetylcholine. Cholinergic relates to the responses in various systems to the neuro-transmitter molecule Acetycholine (ACh). They are the protein that are permanently attached to the biological membrane or the integral membrane protein (IMP). If the set of response is seen where Ach is a normal transmitter it is seen that they are grouped based on nicotinic acetylcholine receptors (nAChR) that respond to nicotine, and muscarinic acetylcholine receptors (mAChR) that bind muscarine. These Nicotine and muscarine are extrinsic molecules that get the same response but with different sensitivity. Drugs that bind to muscarinic receptors are classified based on Agonists (which activate the neuronal receptor and produce a response) Antagonists (which do not activate the receptor and block the agonist binding site) Antagonists are now used to study the drug-receptor binding as they bind with a higher affinity (i.e lower dissociation constant kd) when compared with agonists Pharmacology studies have shown that antagonists have higher affinity but no efficacy to their cognate receptors. They intervene their effect by going and binding to the active site or to allosteric sites on the receptor. They can also go and bind to unique binding sites that do not participate in biological regulation of any receptor activity. The activity that antagonist causes may be reversible or irreversible, depending on the long life of the antagonist-receptor complex. Studies have shown that 3-Quinuclinidyl benzilate (QNB) is a potent muscarinic antagonist in CNS (central nervous system) and peripheral tissues. QNB shows specific binding to the receptor of interest it binds. It can also bind to other sites of the membrane and these can cause changes. We can measure specific binding by filtering radioactive 3H-QNB and then measuring the amount of QNB. To measure non-specific binding, Atropine is used to displace QNB from the specific sites, while the non-specifically bound QNB remains and can be quantified by measuring radioactivity. (Source: Yamamura et al. May 1974) Overview of the experiment QNB is carried out in a radioactive binding assay where the concentration of QNB that is specific bound without atropine and QNB that is non-specifically bound with atropine is measured over successive interval of time. It is allowed to incubate so as for binding site to reach saturation is allowed where the equilibrium is reached. After this any further increase incubation time does not cause the amount of QNB bound to change. This QNB bound to the membrane is measured. By calculating the incubation time, IC50 of atropine is measured by measuring the atropine at which 50% of bound QNB is displaced. Amount of free QNB when 50% of bound QNB is displaced is used to measure the dissociation constant (Kd). Materials and Methods Determination of QNB specific and non-specific binding Two bulk assays was carried out To measure QNB binding (in the presence of water) To measure non specific binding (with the presence of atropine) There were two conical flask taken A and B. Tube A was added with 30 ml of 1.3 nM 3H-QNB and 6ml water. And to the flask B flask B, 30 ml 3H-QNB and 6ml atropine was added. S filter tower is then set with 6 GF/C filters and 4.0 ml of rat membrane was added to each flask and the flask were swirled to mix well. 2ml aliquots from A flask (A1, A2, A3) and (B1, B2, B3) from the B flask were produced and were run through fresh GF/C filters. Each of the filters was then washed to remove mini-vials, and then 5 ml scintillant was added and was left for at least an hour. After a hour the radioactivity was counted in the scintilliant counter. This protocol was repeated for a couple of more time to produce triplicates at the time interval of 10, 20, 30, 45 and 60 min. Determination of IC50 for atropine Five glass test tubes having 1200 μl of distilled water in each was taken. To the test tube 1, 300 μl of 10 10 μM atropine was added and was mixed well. 300 μl of the solution was added to tube 2 and mixed well. The same method is carried out for a series of dilutions to be done in tube 3 to 5. Atropine concentration in each tube is calculated. Seven triplicate tubes (A1, A2, A3…G1, G2, G3) are made each containing 1500 μl of 1.3nM QNB assay and the tubes are mixed well. 300 μl of 10 μM atropine was added to the three tubes of A and three B tubes were added with 300 μl of solution from tube 1. The dilution process was carried out for tubes C, D, E, F from tube 2, tube 3, tube 4 and tube 5 respectively. To tubes G, 300 μl of distilled water was added instead. 200 μl membrane was then added quickly to all the tubes. The 21 tubes were then left for incubation for 45 min and the radioactivity was then measured. Determination of concentration of protein using Lowry Assay Test tubes were prepared that contained 0, 50, 100, 150 and 200 μg BSA (Bovine serum albumin) made up to 1 ml with water. A 6th tube was taken that had 50 μl of membrane that was made up to 1ml with water. 1.5ml of reagent 1 that contains 0.5 ml copper tartrate 50ml alkaline carbonate was added and mixed well and let to stand for 10 min at room temperature. Then 0.3 ml of reagent 2 that contains Commercial Folin-Ciocalteau reagent was added to the tubes and mixed well. The tubes were then left for incubation for 30 min. Absorbance or optical density was read at 660nm. Determination of kd for QNB Eight test tube was taken, four containing low QNB concentration (1.3nM QNB mix) and four tubes containing high QNB concentration (6.5nM QNB mix). Tubes 1 to 4 were added with 7.50 ml, 2.50 ml, 5 ml and 3.2 ml of 6.5 nM QNB mix respectively. Lower concentration of QNB is made by diluting the standard QNB assay mix with NaKP solution. These tubes are labelled 1-8. The solution of tube 1-8, of about 1500 μl each was added to the triplicate tubes (A1, A2, A3, …H1, H2, H3) respectively. Solution of tube 1 is added to tubes A, Tube 2 to B tubes till tube 8 to tubes H. 300 μl water 200 μl membrane was then added to all tubes. For tubes A4-H4, 300 μl Atropine plus (Tube 1-8) respectively plus 200 μl membranes was added. Radioactivity was measured in all tube. A lowry assay was also carried out. RESULT AND DISCUSSION Here in the graph the values are plotted for QNB bound with atropine (with as show in the graph), QNB bound without atropine (Without as shown in the graph) and Corrected vales are obtained by subtracting QNB bound with atropine from the QNB bound without atropine (corrected as shown in the graph ) against time. Here QNB bound without atropine is total amount of QNB bound to the receptor; QNB bound with atropine is the Non-specific binding of QNB to the receptor and corrected is the specific binding of QNB to the receptor. After a particular time of incubation receptors reach equilibrium, where no more binding of QNB takes place to the binding sites. At this point when no more binding of QNB takes place the plateau is formed in the graph showing saturation. This incubation time is approximately “45 min” as shown by the graph reaching the plateau. The graph shows us that with and corrected points of the graph forms a plateau after reaching incubation time of approximately 45 min. If an addition incubation time was taken after 60 min we would have got a plateau for without graph also showing us a plateau. The graph shows that the cmp value increase over time after which when reaching a particular time no more binding occurs thus forming a plateau showing the saturation or equilibrium has reached. Small decline in the graph can be seen at time 30 to 45 min, this could have been due to experimental errors. The errors could have been caused during pipetting, in proper vacuum, formation of bubbles, adding samples properly between time intervals etc. This can be avoided by more careful handling of the instrument and doing a initial check up for errors so as to not cause changes in the experiment’s result. Taking the above data into consideration we have chosen 45 min as incubation time for determining IC50 of atropine. This is because, saturation of binding sites is achieved and no further unbinding of QNB also occurs, as the ‘off-rate’ or reaction constant of QNB unbinding is very low. So there is no further change in the amount of bound QNB and hence this incubation time is considered appropriate. By serial dilution different concentration of atropine was prepared. The graph shows us that the amount to QNB bound to the receptor of the membrane reduces with increase in concentration. This happens because atropine is a competitive binder and binds competitively with specific sites to the receptor. The amount of QNB specifically bound will be inversely proportional with atropine concentration. Half maximal inhibitory concentration (IC50) is a measure of how effective a compound is in inhibiting biological or biochemical function. This is a quantitative measure that let us know how much concentration of the drug or biological substance (inhibitor) is required to inhibit a given biological process by half. So we are calculating the IC50 of atropine to determine its potency. It is calculated by taking atropine concentration at which 50% QNB is displaced. The IC 50 value was found to be 0.0008912 μM. This shows that atropine is a drug with good potency. Ic 50 does not directly discuss the binding constant so we cannot compare the binding affinity of QNB and receptor. Lowary’s assay Lowry’s assay was carried out for determining the concentration of membrane protein. First different concentration of BSA was used and we generated a graph for it, taking concentration and OD. The membrane protein was then checked for absorbance and was found to be 0.322. Using the linear regression equation and the absorbance, concentration of the membrane protein was found to be 0.803 mg/ml. This test was done for another membrane protein sample. The absorbance of the membrane was 0.27. Again using the regression equation and the absorbance, concentration of the membrane protein was found to be 0.293529412 mg/ml. Determination of Kd: Kd is -1/m and was the equation was used is y = -8499.6x – 1.3669. the kd is used to define the affinity between the drug and the protein . the value of Bmax was 0.001161 nm. Binding of QNB and Atropine to Muscarinic Acetylcholine

University of North Dakota National Weather Service & Purposes of Safety Essay

University of North Dakota National Weather Service & Purposes of Safety Essay.

The summary must be no less than 1.5 pages of typed text, double-spaced using a font size of 12.The header information does not count towards text.Top, bottom and side margins will be no larger than 1 inch. The written summary should be typed in a new document and uploaded to the drop box on Blackboard.national economy,
The summary will include details on the various weather parameters provided on the site.What features did the site have?What did you learn?What was your overall general feeling about the site?Did you find the site to be worthwhile and easy to use?In addition, please include two or three images from the site that includes the current date and time stamp.These images can be copied and pasted directly into your word document.These images can include, but not limited to, radar and satellite images, or various weather maps provided on the site.
The images DO NOT count towards the 1.5 pages of text.
Your paper will be graded on content and grammar.This includes, but not limited to: spelling, punctuation, introduction and conclusion paragraphs, sentence structure, general flow of the paper, etc.
The following internet site will be used for the written summary:
Grand Forks NWS Office: https://www.weather.gov/fgf/
University of North Dakota National Weather Service & Purposes of Safety Essay