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Response 3: Compare/Contrast two actor in different time periods.

Response 3: Compare/Contrast two actor in different time periods..

Response 3 is a compare/contrast paper between two actors in different times. Students will watch one of the movies from the list below (years 1980 to now). After watching the actor in the more recent films listed below, the student will then choose an actor/film from an older film provided in the list of films from 1920 to 1979 and watch that film. The objective is to compose a paper comparing and contrasting the two actor’s acting style, and believability. You can do some brief cited research on the actors training if you would like but it is not necessary. The important thing is to focus on what similarities or differences you see in each actor by watching each film. Did you believe or prefer one actor to the other? Again, this is a comparison of the actors not the characters they portray and it is important we see you own reaction in the paper. Minimum word requirement is 600 to 750 words, but feel free to expand if needed. Research is not necessary but if you do include please cite properly!{and check the attachments are examples from the teacher for the this paper you could see it to get an idea about how the paper should look like at the end, so please see them} Timothy Hutton, Mary Tyler Moore, Donald Sutherland: Ordinary People (1980)Sissy Spacek: Coal Miner’s Daughter (1980)Jack Nicholson: The Shining (1980), A Few Good Men (1992), As Good as it Gets (1997), About Schmidt (2002)Robert DeNiro: Raging Bull (1980), The King of Comedy (1982)Peter O’Toole: The Stunt Man (1980), My Favorite Year (1982)Robert Duvall, Michael O’Keefe: The Great Santini (1980)Gene Wilder, Richard Pryor: Stir Crazy (1980)Mel Gibson: Gallipoli (1981), Hamlet (1990), Pocahontas (John Smith) 1995Paul Newman: Absence of Malice (1981), The Verdict (1982), Nobody’s Fool (1994)Robin Williams: The World According To Garp (1982), Awakenings (1990), Aladdin (1992),Good Will Hunting (1997), One Hour Photo (2002)Jerry Lewis: The King of Comedy (1982)Glenn Close: The World According To Garp (1982), Fatal Attraction (1987)Mel Gibson: Gallipoli (1981), Hamlet (1990)Richard Gere, Louis Gossett Jr.: An Officer and a Gentleman (1982)Jessica Lange: Tootsie (1982), Sweet Dreams (1985)William Hurt: Kiss of the Spider Woman (1985), Children of a Lesser God (1986), BroadcastNews (1987), A History of Violence (2005)Debra Winger: An Officer and a Gentleman (1982), Terms of Endearment (1983), Shadowlands(1993), The Lovers (2017)Sean Penn: Fast Times at Ridgemont High (1982), Milk (2008)Meryl Streep: Silkwood (1983) The Bridges of Madison County (1995), Music of The Heart(1999), Adaptation (2002), Julie and Julia (2009), The Iron Lady (2011)Cher: Silkwood (1983), Moonstruck (1987)Shirley MacLaine: Terms of Endearment (1983)Matthew Broderick: War Games (1983), Ferris Bueller’s Day Off (1986)Sally Field: Places in the Heart (1984)Molly Ringwald: Sixteen Candles (1984), Pretty in Pink (1986)Haing S. Ngor: The Killing Fields (1984)Oprah Winfrey: The Color Purple (1985)Whoopi Goldberg: The Color Purple (1985), Ghost (1990)Michael J. Fox: Back to the Future (1985), Casualties of War (1989)Harrison Ford: Witness (1985), Presumed Innocent (1990), Patriot Games (1992)Ed Harris: Sweet Dreams (1985), Pollock (2000), Gone Baby Gone (2007), Kodachrome (2018)Diane Wiest: Hannah and Her Sisters (1986), Parenthood (1989)Dennis Hopper: Blue Velvet (1986), Hoosiers (1986)Michael Douglas: Fatal Attraction (1987)Jennifer Grey: Dirty Dancing (1987)Patrick Swayze: Dirty Dancing (1987), Ghost (1990)Nicholas Cage: Raising Arizona (1987), The Family Man (2000), Adaptation (2002)Holly Hunter: Raising Arizona (1987), Broadcast News (1987), Home for the Holidays (1995), The Big Sick (2017)Tom Hulce, Ray Liotta: Dominick and Eugene (1988)Jodie Foster The Accused (1988), The Silence of the Lambs (1991)Tom Hanks: Big (1988), Philadelphia (1993), Toy Story 3 (2010), Captain Phillips (2013), Saving Mr. Banks (2013), Bridge of Spies (2015)Denzel Washington: Glory (1989), Philadelphia (1993), Flight (2012), Fences (2016)Morgan Freeman: Glory (1989), Driving Mrs. Daisy (1989), The Shawshank Redemption (1994), Gone Baby Gone (2007)Steve Martin, Mary Steenburgen: Parenthood (1989)Daniel Day Lewis: My Left Foot (1989), There Will Be Blood (2007), Lincoln (2012), Phantom Thread (2017)Kevin Costner: The Upside of Anger (2005), Molly’s Game (2017)Bruce Willis: In Country (1989), The Sixth Sense (1999), Unbreakable (2000), Moonrise Kingdom (2012)Jessica Tandy: Driving Miss Daisy (1989), Fried Green Tomatoes (1991)Peter Gallagher, James Spader, Andie MacDowell: Sex Lies and Videotape (1989)Mary Stuart Masterson, Mary Louise Parker: Fried Green Tomatoes (1991)Geena Davis, Susan Sarandon: Thelma and Louise (1991)Kathy Bates: Misery (1990), Fried Green Tomatoes (1991), Primary Colors (1998), About Schmidt (2002)Nick Notle, Kate Nelligan: The Prince of Tides (1991)Anna Chlumsky, Macauley Culkin, Jamie Lee Curtis: My Girl (1991)Kevin Bacon: JFK (1991), The River Wild (1994), My Dog Skip (2000), The Woodsman (2004)Samuel L. Jackson: Jungle Fever (1991), A Time To Kill (1996), Unbreakable (2000)Brad Pitt: A River Runs Through It (1992), The Curious Case of Benjamin Button (2008),Moneyball (2011)Robert Downey Jr.: Chaplin (1992), Home for the Holidays (1995) Zodiac (2007)Clint Eastwood: Unforgiven (1992), The Bridges of Madison County (1995)Marisa Tomei: My Cousin Vinny (1992), The Wrestler (2008)Leonardo DiCaprio: What’s Eating Gilbert Grape (1993), Marvin’s Room (1996), Catch Me if You Can (2002)Anthony Hopkins: Shadowlands (1993)Angela Bassett, Lawrence Fishburne: What’s Love Got to Do With It (1993)Forest Whitaker: The Crying Game (1993), The Last King of Scotland (2006), The Butler (2013)Johnny Depp: What’s Eating Gilbert Grape (1993), Benny and Joon (1993), Finding Neverland (2004)Toni Collette: Muriel’s Wedding (1993), The Sixth Sense (1999), About a Boy (2002), The WayWay Back (2013), Hereditary (2018)Emma Thompson: Sense and Sensibility (1995), Saving Mr. Banks (2013)Will Smith: Six Degrees of Separation (1995), Ali (2001)Kate Winslet: Sense and Sensibility (1995), Little Children (2006), The Reader (2008), Steve Jobs (2015)Irene Badard/Judy Kuhn: Speaking and Singing voice Pocahontas: Pocahontas (1995)Steve Buscemi: Fargo (1996), Ghost World (2001)Francis McDormand: Fargo (1996), Three Billboards Outside of Ebbing, Missouri (2017)Edward Norton: Primal Fear (1996), 25th Hour (2002), Birdman (2014)Tom Cruise: Jerry Maguire (1996), Magnolia (1999)Cuba Gooding Jr.: Jerry Maguire (1996)William H. Macy: Fargo (1996), Boogie Nights (1997), Magnolia (1999)Natalie Portman: Beautiful Girls (1996), Closer (2003). Black Swan (2010), Jackie (2016)Julianne Moore: Boogie Nights (1997), The Hours (2002), The Kids are Alright (2010), Don Jon(2013), Still Alice (2014)Greg Kinnear, Helen Hunt: As Good as it Gets (1997)John C. Reilly: Boogie Nights (1997)Matt Damon: Good Will Hunting (1997), The Talented Mr. Ripley (1999)Burt Reynolds Marc Walhberg: Boogie Nights (1997)Angelina Jolie: Playing By Heart (1998), Changeling (2008)Annette Benning: American Beauty (1999), The Kids are All Right (2010)Hugh Grant: Notting Hill (1999), About a Boy (2002), Music and Lyrics (2007)Jude Law: The Talented Mr. Ripley (1999), Closer (2003), Side Effects (2013)Russell Crowe: The Insider (1999), A Beautiful Mind (2001), 3:10 to Yuma (2007)Christian Bale: American Psycho (2000), 3:10 to Yuma (2007), The Fighter (2010), American Hustle (2013), The Big Short (2015)Julia Roberts: Notting Hill (1999), Erin Brockovich (2000) Closer (2003), August Osage County(2013)Javier Bardem: Before Night Falls (2000), Vicki Cristina Barcelona (2008), No Country for OldMen (2007)Laura Linney: You Can Count on Me (2000), Mystic River (2003), Kinsey (2004), The Squid and the Whale (2005)Mark Ruffalo: You Can Count on Me (2000), Zodiac (2007), The Kids are Alright (2010) Foxcatcher (2014)Cate Blanchette: The Gift (2000), Notes on a Scandal (2006), I’m Not There (2007), Blue Jasmine (2013), Carol (2015)Ellen Burstyn: Requiem for A Dream (2000)Ewan McGregor: Moulin Rouge (2001), I Love You Phillip B. Morris (2009) Beginners (2010), The Impossible (2012)Nicole Kidman: Moulin Rouge (2001), The Others (2002), The Hours (2002), Rabbit Hole (2010)Ryan Gosling: The Believer (2001), Half Nelson (2006), Lars and the Real Girl (2007), Blue Valentine (2010), The Ides of March (2011), Drive (2011), The Place Beyond the Pines (2013), The Big Short (2015)Clive Owen: Gosford Park (2001), Closer (2003)Helen Mirren: Gosford Park (2001), The Queen (2006), Hitchcock (2012), Woman in Gold (2015), Trumbo (2015)Renee Zellweger: Bridget Jones Diary (2001), My One and Only (2009)Chiwetel Ejiofor: Dirty Pretty Things (2002), Talk to Me (2007), 12 Years a Slave (2013)Jason Statham: The Transporter (2002), Crank (2006)Sam Rockwell: Confessions of a Dangerous Mind (2002), Joshua (2007), Moon (2009), The Way Way Back (2013), Three Billboards Outside Ebbing, Missouri (2017)Naomi Watts: 21 Grams (2003), The Impossible (2012), St Vincent (2014)Charlize Theron: Monster (2003), Young Adult (2011)Rachel Weisz: The Shape of Things (2003), The Constant Gardener (2005)Paul Rudd: The Shape of Things (2003)Don Cheadle, Sophia Okonedo: Hotel Rwanda (2004)Jamie Foxx: Ray (2004), Collateral (2004)Paul Giamatti: Sideways (2004), Barney’s Version (2010), The Ides of March (2011), Saving Mr. Banks (2013). Love and Mercy (2015)Thomas Hayden Church, Virginia Madsen, Sandra Oh: Sideways (2004)Sarala: Water (2005)Amy Adams: Junebug (2005), Julie and Julia (2009), The Fighter (2010), American Hustle (2013), Her (2013), Arrival (2016)Jake Gyllenhaal: Brokeback Mountain (2005), Love and Other Drugs (2010), Nightcrawler(2014), Stronger (2017)Heath Ledger: Brokeback Mountain (2005)Viggo Mortenson: A History of Violence (2005), Captain Fantastic (2016)Felicity Hoffman: Transamerica (2005)Mads Mikkelson: After the Wedding (2006), The Hunt (2012)Philip Seymour Hoffman: Capote (2005), The Ides of March (2011)Michael Sheen: The Queen (2006), Frost/Nixon (2008)Penelope Cruz: Volver (2006), Vicki Cristina Barcelona (2008)Adrianna Barraza: Babel (2006)Judi Dench: Notes on a Scandal (2006), Philomena (2013)Will Ferrell: Stranger than Fiction (2006), Blades of Glory (2007)Ben Affleck: Hollywoodland (2006), Gone Girl (2014)Jennifer Hudson, Beyonce, Eddie Murphy: Dreamgirls (2006)Taraji Henson: Talk to Me (2007), The Curious Case of Benjamin Button (2008)Casey Affleck, Amy Ryan: Gone Baby Gone (2007)Ryan Reynolds: The Nines (2007), Buried (2010), Definitely Maybe (2008), Mississippi Grind(2015)Marion Cotillard: La Vie En Rose (2007), Rust and Bone (2012), Two Days, One Night (2014)Isla Fisher: Definitely Maybe (2008)Colin Ferrell: In Bruges (2008)James Franco: Milk (2008), 127 Hours (2010) Hiam Abass: The Visitor (2008)Richard Jenkins: The Visitor (2008), The Shape of Water (2016)Josh Brolin: Milk (2008)Mickey Rourke: The Wrestler (2008)Frank Langella: Frost/Nixon (2008)Viola Davis: Doubt (2008), Fences (2016)Red West, Souleymane Sy Savane: Goodbye Solo (2009)Jeremy Renner: The Hurt Locker (2009), The Town (2010), American Hustle (2013), Wind River(2017)Jim Carrey: I Love You Phillip B. Morris (2009)Anthony Mackie: The Hurt Locker (2009)Maya Rudolph,: Away We Go (2009)John Krasinski: Away We Go (2009), A Quiet Place (2018)George Clooney: Up in the Air (2009), The Ides of March (2011) The Descendants (2011)Vera Farmiga, Anna Kendrick: Up in the Air (2009),Jim Carrey: I Love You Phillip B Morris (2009)Gabourey Sidibe, Mo’Nique: Precious (2009)Carey Mulligan: An Education (2009), Shame (2011), Far from the Madding Crowd (2015)Colin Firth: Bridget Jones Diary (2002), A Single Man (2009), The King’s Speech (2010)Jeff Bridges: Crazy Heart (2009), Hell or High Water (2016)Anne Hathaway: Love and Other Drugs (2010), Les Miserables (2012)Mark Wahlberg, Melissa Leo: The Fighter (2010)Annette Bening: The Kids are Alright (2010)Tim Allen: Toy Story 3 (2010)Barbara Hershey: Black Swan (2010)Geoffrey Rush: The King’s Speech (2010)Miles Teller: Rabbit Hole (2010), The Spectacular Now (2013), Whiplash (2014)Michelle Williams: Blue Valentine (2010), My Week with Marilyn (2011), All the Money in theWorld (2017)Jonah Hill: Moneyball (2011)Owen Wilson, Corey Stoll: Midnight in Paris (2011)Demian Bichar: A Better Life (2011)Christopher Plummer: Beginners (2010)Tom Hardy: Warrior (2011), Locke (2014)Joel Edgerton, Nick Nolte: Warrior (2011)Rooney Mara: The Girl with the Dragon Tattoo (2011), Side Effects (2013), Carol (2015)Patton Oswald: Young Adult (2011)Michael Fessbender: Shame (2011), 12 Years a Slave (2013), Steve Jobs (2015)Bryan Cranston: Drive (2011), Argo (2012) Trumbo (2015)Shailene Woodley: The Descendants (2011), The Spectacular Now (2013)Isiah Whitlock Jr.: Cedar Rapids (2011)Joseph Gordon-Levitt: 50/50 (2011), Don Jon (2013)Seth Rogen, Anna Kendrick, Anjelica Huston: 50/50 (2011)Ed Helms: Cedar Rapids (2011), Jeff Who Lives at Home (2012)Jason Segel: Jeff Who Lives at Home (2012)Matthew McConaughey: Magic Mike (2012), Mud (2013), Dallas Buyers Club (2013)Bradley Cooper: Silver Linings Playbook (2012), The Place Beyond the Pines (2013), American Hustle (2013)Jennifer Lawrence: Silver Linings Playbook (2012), American Hustle (2013), Mother (2017)Logan Lorman, Ezra Miller, Emma Watson: The Perks of Being a Wallflower (2012)John Hawkes: The Sessions (2012)Matthias Schoenaerts: Rust and Bone (2012), Far from the Madding Crowd (2015)John Goodman, Bryon Cranston: Argo (2012)Quvenzhane Wallis: Beasts of the Southern Wild (2012)Hugh Jackman: Les Miserables (2012)Eddie Redmayme: Les Miserables (2012), The Theory of Everything (2014), The Danish Girl(2015)Tom Holland: The Impossible (2012)Sharni Vinson: You’re Next (2013)Jared Leto: Dallas Buyers Club (2013)Julia Louis Dreyfus, James Gandolfini: Enough Said (2013)Bruce Dern, Will Forte, June Squibb: Nebraska (2013)Robert Redford: All is Lost (2013)Oprah Winfrey:The Butler (2013)Lupita N’yongo: 12 Years a Slave (2013)Joaquin Phoenix, Scarlette Johansson: Her (2013)Steve Coogan: Philomena (2013)Reece Witherspoon: Mud (2013), Wild (2014)Ralph Fiennes, Tony Revolori: The Grand Budapest Hotel (2014)Felicity Jones: The Theory of Everything (2014)Steve Carrell: Foxcatcher (2014), The Big Short (2015), Battle of the Sexes (2017)Channing Tatum: Foxcatcher (2014)Kristin Wiig, Bill Hader: The Skeleton Twins (2014)Michael Keaton: Birdman (2014), Spotlight (2015)Emma Stone: Birdman (2014), Battle of the Sexes (2017)Patricia Arquette: Boyhood (2014)Ethan Hawke: Boyhood (2014), First Reformed (2018)J.K. Simmons; Whiplash (2014)Rosemund Pike: Gone Girl (2014)David Oyelowo, Carmen Ejogo: Selma (2014)Michael B Jordan: Creed (2015)Josh Lucas, Stephen Plunkett: The Mend (2015)Charlotte Rampling, Tom Courtenay: 45 Years (2015)Brie Larson, Jacob Trembley: Room (2015)Ben Mendelsohn: Mississippi Grind (2015)Paul Dano, Elizabeth Banks: Love and Mercy (2015)Alicia Vikander: The Danish Girl (2015)Sylvester Stallone: Creed (2015)Saoirse Ronan: Brooklyn (2015), Lady Bird (2017)Mark Rylance: Bridge of Spies (2015)Rachel McAdams: Spotlight (2015)Chris Pine, Ben Foster: Hell or High Water (2016)Isabelle Huppert: Elle (2016)Mahershala Ali, Naomie Harris, Andre Holland (adult Kevin), Trevante Rhodes (adult Chiron), Alex Hibbert(child Chiron): Moonlight (2016)Ruth Negga: Loving (2016)Tika Sumpter, Parker Sawyers: Southside with You (2016)Andrew Garfield: Hacksaw Ridge (2016)Hailee Steinfeld,: Edge of Seventeen (2016)Woody Harrelson: Edge of Seventeen (2016), Three Billboards Outside of Ebbing, Missouri(2017)James McAvoy: Split (2017)Mark Rylance: Dunkirk (2017)Fionn Whitehead:Dunkirk (2017)Laurie Metcalf: Lady Bird (2017)Tatiana Maslany: Stronger (2017)Michelle Pheiffer: Mother (2017)Elizabeth Oleon, Gil Birmingham: Wind River (2017)Gary Oldman: The Darkest Hour (2017)Ben Stiller: Brad’s Status (2017)Jessica Chastain: Molly’s Game (2017)Mary J. Blige: Mudbound (2017)Alison Janney, Margot Robbie: I Tonya (2017)Kumail Najiani, Ray Romano, Zoe Kazan: The Big Sick (2017)Sally Hawkins, Octavia Spencer: The Shape of Water (2017)Dan Stevens: The Man Who Invented Christmas (2017)Vicky Krieps, Lesley Manville: Phantom Thread (2017)Jason Sudaris: Kodachrome (2018)Emily Blunt, Millicent Simmonds: A Quiet Place (2018)Alex Wolff: Hereditary (2018)Nick Robinson: Love Simon (2018)Charlie Plummer: Lean on Pete (2018)From 1979 to 1920Lillian Gish: Broken Blossoms (1919), Way Down East (1920)Max Schreck: Nosferatu (1922)Harold Lloyd: Safety Last (1923)Charlie Chaplin: The Kid (1921), The Gold Rush (1925), City Lights (1931), Modern Times (1936), The Great Dictator (1940)Janet Gaynor: Sunrise: A Song of Two Humans (1927), A Star Is Born (1937)Buster Keaton: Steamboat Bill Jr. (1928), The General (1927)Louise Brooks: Diary of a Lost Girl (1929), Pandora’s Box (1929)Bela Lugosi: Dracula (1931)Boris Karloff: Frankenstein (1931)Joan Crawford: Grand Hotel (1932)Paul Muni: I Am A Fugitive from a Chain Gang (1932), Scarface (1932)James Cagney: Public Enemy (1931), Angels with Dirty Faces (1938), Yankee Doodle Dandy(1942), White Heat (1949)Greta Garbo: Grand Hotel (1932), Ninotchka (1939)Mae West: I’m No Angel (1933), She Done Him Wrong (1933)Clark Gable: It Happened One Night (1934), Gone With the Wind (1939)Claudette Colbert: It Happened One Night (1934), Imitation of Life (1934), Since You Went Away (1944)Louise Beavers: Imitation of Life (1934)Shirley Temple: Bright Eyes (1934), The Little Colonel (1935), Heidi (1937), The Little Princess(1939)The Marx Brothers: Duck Soup (1933), A Night at The Opera (1935)Gary Cooper: Mr. Deeds Goes to Town (1936), The Pride of the Yankees (1942)Fred Astaire: Top Hat (1935), Swing Time (1936)Ginger Rogers: Top Hat (1935), Swing Time (1936), Stage Door (1937), I’ll Be Seeing You (1944)Fredric March: A Star Is Born (1937)Robert Donat: Goodbye Mr. Chips (1939)Charles Laughton: The Hunchback of Norte Dame (1939)John Wayne: Stagecoach (1939), Red River (1948)Katharine Hepburn: Bringing Up Baby (1938), The Philadelphia Story (1940), Adam’s Rib (1949)Errol Flynn: The Adventures of Robin Hood (1938), Gentleman Jim (1942)Cary Grant: Bringing Up Baby (1938), The Philadelphia Story (1940), Notorious (1946)James Stewart: Mr. Smith Goes to Washington (1939), The Shop Around the Corner (1940), The Philadelphia Story (1940), Destry Rides Again (1939)Merle Oberon: Wuthering Heights (1939)Lawrence Olivier: Wuthering Heights (1939), Rebecca (1940)Vivien Leigh: Gone With the Wind (1939)Hattie McDaniel: Gone With the Wind (1939) Since You Went Away (1944)Marlene Dietrich: Destry Rides Again (1939)Burgess Meredith, Lon Chaney Jr: Of Mice and Men (1939)Bette Davis: Dark Victory (1939), The Little Foxes (1941), Now Voyager (1942)Margaret Sullavan: The Shop Around the Corner (1940)Irene Dunne: My Favorite Wife (1940), I Remember Mama (1948)Joan Fontaine, Judith Anderson: Rebecca (1940)Humphrey Bogart: The Maltese Falcon (1941), To Have and Have Not (1944), Treasure of the Sierra Madre (1948)Ingrid Bergman: Spellbound (1945), Notorious (1946)Henry Fonda: The Grapes of Wrath (1940), My Darling Clementine (1946)Orson Welles: Citizen Kane (1941), The Third Man (1949)Agnes Moorehead: Citizen Kane (1941), Since You Went Away (1944), Johnny Belinda (1948)Greer Garson, Teresa Wright: Mrs Miniver (1942)Ethel Waters: Cabin in the Sky (1943)Gene Tierney: Laura (1944), Leave Her to Heaven (1945)Lena Horne: Stormy Weather (1944)Gregory Peck: Spellbound (1945), Gentleman’s Agreement (1947)Edward G. Robinson: Double Indemnity (1944), Key Largo (1948)Lauren Bacall: To Have and Have Not (1944), The Big Sleep (1946)Barbara Stanwyck: Double Indemnity (1944), Christmas in Connecticut (1945)Robert Mitchum: Out of the Past (1947)Edmund Gwenn: Miracle on 34th Street (1947)Barbara Bel Geddes: I Remember Mama (1948)Judy Garland: Easter Parade (1948)John Wayne: Stagecoach (1939), Red River (1948)Montgomery Clift: Red River (1948), The Heiress (1949)Jane Wyman: Johnny Belinda (1948)Spencer Tracy: Adam’s Rib (1949)Olivia de Havilland: The Heiress (1949)James Edwards: The Steel Helmet (1951)Ava Gardner: Showboat (1951)Vivien Leigh: A Streetcar Named Desire (1951)Marlon Brando: A Streetcar Named Desire (1951), On the Waterfront (1954)Ethel Waters A Member of the Wedding (1952)Donna Reed, Burt Lancaster: From Here to Eternity (1953)Audrey Hepburn: Roman Holiday (1953), Sabrina (1954)Montgomery Clift: From Here to Eternity (1953), The Misfits (1961)James Stewart: Rear Window (1954), Vertigo (1958), Anatomy of a Murder (1959)Dorothy Dandridge Carmen Jones (1954)Pearl Bailey: Carmen Jones (1954), Porgy and Bess (1959)Judy Garland: A Star is Born (1954)Grace Kelly: Rear Window (1954), Dial M for Murder (1955)Ernest Borgnine: Marty (1955)James Dean: Rebel Without a Cause (1955), East of Eden (1955), Giant (1956)Robert Mitchum: The Night of the Hunter (1955)Yul Brenner, Deborah Kerr: The King and I (1956)Rita Moreno: The King and I (1956), West Side Story (1961)Dorothy Malone, Robert Stack: Written on the Wind (1956)Rock Hudson: Giant (1956), Pillow Talk (1959), Seconds (1966)Paul Newman: Cat on a Hot Tin Roof (1958)Elizabeth Taylor: Cat on a Hot Tin Roof (1958), Suddenly Last Summer (1959), Who’s Afraid of Virginia Woolf? (1966)Juanita Moore: Imitation of Life (1959)Doris Day: Pillow Talk (1959)Kim Novak: Vertigo (1958)George C. Scott: Anatomy of a Murder (1959), Dr. Strangelove (1964), Patton (1970)Lee Remick: Anatomy of a Murder (1959)Tony Curtis, Marilyn Monroe: Some Like It Hot (1959)Jack Lemmon: Some Like It Hot (1959), The Apartment (1960)Shirley MacLaine: The Apartment (1960)Anthony Perkins, Janet Leigh: Psycho (1960)Burt Lancaster, Shirley Jones: Elmer Gantry (1960)Ruby Dee: A Raisin in the Sun (1961)Sidney Poitier: A Raisin in the Sun (1961), A Patch of Blue (1965), Guess Who’s Coming To Dinner (1967)Sophia Loren: Two Women (1961)Natalie Wood, George Chakiris: West Side Story (1961)Audrey Hepburn: Roman Holiday (1953), Sabrina (1955)Shelley Winters: Lolita (1962), A Patch of Blue (1965)Angela Lansbury: The Manchurian Candidate (1962)Shelley Winters: Lolita (1962), A Patch of Blue (1965)Peter Sellers: Lolita (1962) Dr. Strangelove (1964)Richard Burton: The Night of the Iguana (1964), Who’s Afraid of Virginia Woolf? (1966)Rod Steiger: The Pawnbroker (1965)Brock Peters: The Pawnbroker (1965)Michael Caine: Alfie (1966)Robert Blake: In Cold Blood (1967)Spencer Tracy/Katharine Hepburn: Guess Who’s Coming To Dinner (1967)Warren Beatty: Bonnie and Clyde (1967)Faye Dunaway: Bonnie and Clyde (1967), Chinatown (1967)Dustin Hoffman: The Graduate (1967), Midnight Cowboy (1969) Little Big Man (1970), Straw Dogs (1971), All the President’s Men (1976), Marathon Man (1976)Anne Bancroft: The Graduate (1967)Paul Newman: Cool Hand Luke (1967), Butch Cassidy and The Sundance Kid (1969)Barbra Streisand: Funny Girl (1968), The Way We Were (1973)Mia Farrow, Ruth Gordon: Rosemary’s Baby (1968)Robert Redford: Butch Cassidy and The Sundance Kid (1969), The Way We Were (1973), Three Days of the Condor (1975), All the President’s Men (1976)Paul Newman: Cool Hand Luke (1967), Butch Cassidy and The Sundance Kid (1969)Liza Minelli: The Sterile Cuckoo (1969), Cabaret (1972)Jack Nicholson: Five Easy Pieces (1970), Chinatown (1974), One Flew Over the Cuckoo’s Nest(1975)Maggie Smith: The Prime of Miss Jean Brodie (1969)Jon Voight: Midnight Cowboy (1969), Deliverance (1972)Chief Dan George: Little Big Man (1970)Elliott Gould, Donald Sutherland: M*A*S*H (1970)Ali MacGraw, Ryan O’Neal: Love Story (1970)Malcolm MacDowell: A Clockwork Orange (1971)Jane Fonda: Klute (1971), Fun with Dick and Jane (1977)Clint Eastwood: Dirty Harry (1971), The Outlaw Josey Wales (1976)Topol: Fiddler on the Roof (1971)Cloris Leachman, Jeff Bridges, Timothy Bottoms: The Last Picture Show (1971)Joel Grey: Cabaret (1972)Burt Reynolds: Deliverance (1972), Smokey and the Bandit (1977)Al Pacino: The Godfather (1972), Serpico (1973), Dog Day Afternoon (1975)Cicely Tyson, Paul Winfield: Sounder (1972)Linda Blair: The Exorcist (1973)Ellen Burstyn: The Exorcist (1974), Alice Doesn’t Live Here Anymore (1974)Sissy Spacek: Badlands (1973)Martin Sheen: Badlands (1973), Apocalypse Now (1979)Robert Redford: Butch Cassidy and The Sundance Kid (1969), The Way We Were (1973), Three Days of the Condor (1975), All the President’s Men (1976)Louise Fletcher: One Flew Over the Cuckoo’s Nest (1975)Cleavon Little: Blazing Saddles (1974)Gene Hackman: The Conversation (1974)Ellen Burstyn: The Exorcist (1973), Alice Doesn’t Live Here Anymore (1974)Gene Wilder, Richard Pryor: Silver Streak (1976)Chief Dan George: The Outlaw Josey Wales (1976)Gregory Peck, Lee Remick: The Omen (1976)Robert DeNiro: Taxi Driver (1976), The Deer Hunter (1978)George Segal: Fun with Dick and Jane (1977)John Travolta: Saturday Night Fever (1977)Genevieve Bujold: Coma (1978)Jill Clayburgh: An Unmarried Woman (1978)Christopher Walken: The Deer Hunter (1978)Robert Duvall: Apocalypse Now (1979)Meryl Streep, Justin Henry: Kramer vs Kramer (1979)Sigourney Weaver: Alien (1979)Sally Field: Norma Rae (1979)
Response 3: Compare/Contrast two actor in different time periods.

Academy of Careers and Technology Advancement of Technology and E Commerce Discussion.

appos is a leading e-commerce company whose core principle is “to live and deliver WOW”, which is how it grew to be a leader in the online market. A customer service representative will do anything — even spend Zappos money — to “wow” customers, even if it means flying to a customer’s home to return jewelry that got shipped to the company by accident!Refer back to page 130 of Chapter 8 about “REWARDING CSRs” and then view the following video: “Jewelry | Zappos True Customer Story” Answer the following questions in your own words.
Name three things about the incident in the video that made it exceptional.

What is the best example of “exceptional” Customer Service that you have experienced? How did you feel about the business afterward?

What are some of the challenges that prevent more companies from doing things like Zappos?
Academy of Careers and Technology Advancement of Technology and E Commerce Discussion

Individual project for Xfinity/Comcast, management homework help.

I am going to try this again. No plagarism…if you use outside sources the plagiarism needs to be under 20%THE COMPANY I CHOSE IS COMCAST/XFINITY-PLEASE READ THE INSTRUCTIONS CAREFULLY AND DO NOT PLAGIARIZE….THIS WILL BE PUT THROUGH TURNITINSelect a U.S. multinational company, and respond to the following questions:In terms of currency denomination, describe how the firm prices its revenues and costs.For multinational enterprises (MNEs) with multiple foreign operations, consider any 2 of those operations and the contribution they are making to the parent firm’s profits.What means do they use to hedge against exchange rate risk?Using this information, what do you think would be the effect of increases or decreases in the dollar’s exchange value on the firm’s profitability?Be sure to show all applicable work.Present your findings as a Word document of 7–10 pages formatted in APA style. Include 5–7 academic, peer-reviewed references that are relevant to and support the deliverable.Submitting your assignment in APA format means, at a minimum, you will need the following:Title page: Remember the running head AND THE TITLE IN ALL CAPITALS.Abstract: This is a summary of your paper—not an introduction. Begin writing in third-person voice.Body: The body of your paper begins on the page following the title page and abstract page and must be double-spaced (be careful not to triple- or quadruple-space between paragraphs). The type face should be 12-pt. Times Roman or 12-pt. Courier in regular black type. Do not use color, bold type, or italics except as required for APA-level headings and references. The deliverable length of the body of your paper for this assignment is 3–4 pages. In-body academic citations to support your decisions and analysis are required. A variety of academic sources is encouraged.Reference page: References that align with your in-body academic sources are listed on the final page of your paper. The references must be in APA format using appropriate spacing, hang indention, italics, and upper- and lowercase usage as appropriate for the type of resource used. Remember, the Reference page is not a bibliography but a further listing of the abbreviated in-body citations used in the paper. Every referenced item must have a corresponding in-body citation.This assignment will be assessed using the rubric provided here.Please submit your assignment.For assistance with your assignment, please use your text, Web resources, and all course materials.
Individual project for Xfinity/Comcast, management homework help

SYG 2000 Prairie State College Cultural Norms and COVID 19 Essay

SYG 2000 Prairie State College Cultural Norms and COVID 19 Essay.

must consist of of 1000-1500 wordsits based off chapter 3 of this book https://openstax.org/books/introduction-sociology-2e/pages/3-1-what-is-cultureFor your paper, consider the following questions (and suggestions):
● What cultural norms and symbolic gestures have changed as a result of society’s
response to the COVID-19 pandemic? I encourage you to have discussions with family
members and friends about this question. Also, if you are out in public (and wisely
following the new rules), apply the sociological method of observation, which you
learned about in Chapter 2, and take notes (at least mental notes) on the patterns of
behavior that you observe. I see this part of the paper as your first body paragraph, with
a focus on description of your observations.
● How have these changing cultural norms affected your daily life experiences? How have
you been adjusting your behavior (whether by choice or by moral obligation)? For this
part of the paper, I want you to reflect on how these changes have affected you; as you
write on this, it is fine to use the first-person, “I,” in your discussion. I see this part of the
paper as your second body paragraph, with a focus on personal reflection.
● Do you think this national mobilization, really global mobilization, to combat the
pandemic will have a long-term impact on the way we live our lives, on the core values
and norms that define culture in the United States? If so, how? If not, why not? This part
of the paper asks you to reconnect with the broader sociological context for your
observations and reflections. I see this part of your paper as either your third body
paragraph or a concluding paragraph, with a focus on sociological analysis.
In addition to my suggestions above, please make sure to design an introductory paragraph that
sets the stage for your discussion by establishing the sociological context of the assignment.
Like the concluding paragraph, the intro should be sociological and analytical in tone. It is fine to
borrow some of the broad ideas I lay out in the Sociological Background section of this
assignment, but make sure to establish your own voice as you make concrete connections to
the sociological perspective. I look forward to reading your papers.
SYG 2000 Prairie State College Cultural Norms and COVID 19 Essay

help me in my duty mgt 201

assignment writing services help me in my duty mgt 201. I need an explanation for this Marketing question to help me study.

The Assignment must be submitted on Blackboard (WORD format only) via allocated folder.
Assignments submitted through email will not be accepted.
Students are advised to make their work clear and well presented, marks may be reduced for poor presentation. This includes filling your information on the cover page.
Students must mention question number clearly in their answer.
Late submission will NOT be accepted.
Avoid plagiarism, the work should be in your own words, copying from students or other resources without proper referencing will result in ZERO marks. No exceptions.
All answered must be typed using Times New Roman (size 12, double-spaced) font. No pictures containing text will be accepted and will be considered plagiarism).
Submissions without this cover page will NOT be accepted.

Assignment Question(s):(Marks- 05)
Critical Thinking
The market for young people’s food products has increased considerably in recent years. As a result, children have become high-potential customers and are now the focus of intense and specialized marketing and advertising efforts.
Children and adolescents have become attractive consumers and influencers: they have an increasing influence on their family’s purchases. Children represent an important target audience for marketers because they have their own purchasing power, influence their parents’ purchasing decisions, and are the consumers of tomorrow. Advertisers have an interest in seducing them from an early age. Thus, to be sure to attract young people, companies opt for a combination of different approaches and channels such as television advertising, contests and games, toys, the use of popular characters, the use of various attractive colors, school marketing, Internet and branded products etc.
Food and beverages products that target children have increased and these products are dominated by foods that are high in calories, sugars, salt, fat, low in nutrients and therefore not compatible with national dietary recommendations.
Mr. Fahmy has already a business in the food sector and wants to open a new subsidiary specializing in natural and organic products for children. The goal is to provide healthy, nutritious food and use healthier ingredients.
Questions
1. Help Mr. Fahmy define his overall goals, objectives and strategies for his new business in the context of his mission and purpose.
2. Briefly describe the choices that Mr. Fahmy can make for each of the 4 Ps of the marketing mix.
3. Identify the target market and describe how Mr. Fahmy’s activities will respond better than the competition to the needs of the consumer. (List consumer expectations for the product)
4. Describe the type of promotional methods that you recommend to Mr. Fahmy for the promotion of his product line. (Identify techniques such as word of mouth, personal sales, direct marketing, sales promotion, etc., on television, radio, social media, and newspapers).
5. Why is it important for a media planner to consider how different types of media could work together on a media plan?

8 reference at least

help me in my duty mgt 201

Molecular Weight Of Unknown Protein

Molecular Weight Of Unknown Protein. Abstract SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is used to separate protein molecules based on size. By using sodium dodecyl sulphate (SDS) and a gel made from acrylamide, protein shape, structure and charge no longer become factors as proteins migrate on to gels and protein bands are only affected by size. Together with Western Blotting (WB) both common laboratory techniques, they are used to further determine the presence of a given protein. Using this knowledge, this report will look at both techniques and also discuss how both techniques can determine the molecular weight of an unknown protein. Protein standards and an unknown protein sample will be prepared for SDS-PAGE using both a resolving and a stacker gel, and for WB analysis. The known protein standards are identified and act as markers for the unknown sample. The unknown sample migrates to a similar distance as the β- Galactosidase protein, as well as their Molecular Weights (MW) being very close together. It was concluded therefore, that the unknown protein was β- Galactosidase, but also both techniques, SDS-PAGE and WB are useful tools when determining the molecular weights of unknowns, but also identifying them too. Both variations of the same purpose were successful, however, it can be said that the SDS-PAGE can give a more quantitative and definite outcome, based upon the results in this report. Introduction SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is the most commonly used laboratory technique to separate proteins. It involves applying an electric current to a polyacrylamide gel matrix, allowing the proteins to migrate through the matrix. However, in order for proteins to migrate onto the gel at a similar rate, or at all, the use of the detergent SDS is required in order to denature the proteins, which do so in the presence of SDS. Proteins become negatively charged, as they bind to SDS, forming a protein-detergent complex. Approximately 1.4 g of SDS becomes bound to per gram of protein, giving the denatured proteins a similar charge to mass ratio. Therefore, since the denatured proteins no longer have a complex tertiary shape, but form similar rod-like structures and they all have a negative charge, differences in shape and charge are no longer factors for separation in the gel. Consequently, the rate at which the SDS coated proteins migrate in the gel is purely dependent on size. Previous studies have involved using SDS-PAGE to separate urinary proteins by MW (1). The SDS-PAGE in this experiment was also carried out following the procedure described by Laemmli, who carried out an experiment using a stacking gel and SDS (2). Laemmli’s system has shown to be the most popular discontinuous buffer system. SDS-PAGE involves the use of discontinuous gels, consisting of a resolving or separating gel as well as a stacking gel. The resolving lower gel is where the proteins migrate to and separate into different sizes. The quantity of acrylamide in the resolving gel defines how well the separation takes place since higher concentrations of acrylamide restrict the mobility of proteins. The stacking upper gel is where the proteins are loaded and acts to concentrate large sample volumes, producing better band resolutions, compared to a gel with no stack. The difference in pH is one of the reasons why the proteins move from the stacking gel to the resolving gel, since the pH causes the molecules that were blocking the proteins moving through the stack, to separate, therefore freeing the proteins to move down to the resolving gel. A molecular weight marker such as a known protein or standard that produces bands of known size, migrates to the resolving gel, and is used to help identify unknown proteins (1). Western Blotting (WB) is a powerful and important procedure which follows successful electrophoresis for the detection of proteins, specifically those in low quantities (3). WB allows the transfer of proteins to a nitrocellulose membrane. Using the same principles as SDS-PAGE, an electric current is also used, but is applied at 90° to the gel, allowing the proteins to migrate out of the gel onto the membrane. WB can now begin to detect a target protein in a sample by using an antibody specific to that protein. Thus, revolutionising the immunological field, since prior to WB, the separated probes in the gel were difficult to access with molecular probes (3). Therefore in this experiment, it was sought to determine the MW and identity of the Unknown protein using the two different techniques mentioned earlier, SDS-PAGE and Western Blotting. Methods SDS-PAGE Preparation A 7.5% resolving gel monomer solution was prepared combining 4.85ml of Distilled Water; 2.5ml of 1.5M Tris-HCL (pH8.8); 100µl of 10% (w/v) SDS stock and 2.5ml of 30% Acrylamide/Bis. A comb is placed in the assembled gel sandwich and then carefully removed. 50µl of 10% ammonium persulfate and 5µl of TEMED were added to the monomer solution and mixed well, avoiding aeration. The solution was then poured to within 1cm of the comb. The gel was overlain butanol and left to polymerise. These steps to create a discontinuous buffer system are that of Laemmli (2), and were already completed by staff. The preparation of the 4% stacking gel was performed by the students, and involved combining 6.1ml of Distilled water; 2.5ml of 0.5M Tris-HC1 (pH 6.8); 100µl of 10% SDS; 1.3ml of 30% Acrylamide/Bis; 50µl of 10% ammonium persulfate and 10µ of TEMED. After the butanol had been removed from the top of the gel and the gel had been washed, it was filled with stacking gel. The comb was inserted and left to polymerise for 45-60 minutes. After this time, the comb was removed and the gel rinsed through with distilled water. Standard Sample and Unknown Protein Sample Preparation 2 tubes containing pre-stained protein standards mixed with sample buffer (0.01M phosphate (pH 7.2) containing 1% SDS, 10% glycerol, 0.002% bromophenol blue and 0.01M mercaptoethanol) in one, and pre-stained Unknown protein sample mixed with the same sample buffer in the other were given and 10µl of each sample were removed and placed in separate micro centrifuge tubes. The tubes were incubated in a boiling water bath for 3 minutes and then the contents of the tube containing the protein samples was added to 1 well, whilst the contents of the tube containing the unknown protein was added to another well. The gels were run at 200V, until the bromophenol blue band was 1cm from the bottom of the gel, which took approx. 55 minutes. After the electrophoresis was complete, the lining membrane was removed from the sandwich and the glass plates were lifted away from the gel. The gels were measured and then placed in transfer buffer for 10 minutes. The proteins standards and their molecular weights were identified, calculated and given to the students. Western Blotting Nitrocellulose paper, scotch-brite fibre pads and filter paper, were cut to the size of the gel, and the latter two were saturated with transfer buffer. The nitrocellulose should be touched to the buffer and allowed to wet by capillary action. The Trans-Blot cell assembly was set up by placing a fibre pad onto the grey side of the gel holder, followed by a saturated piece of filter paper; the pre-equilibrated SDS gel and the nitrocellulose sheet. Air bubbles were removed before and after a further piece of saturated filter paper was placed on top of the nitrocellulose. Finally, a saturated fibre pad was placed on top of the filter paper. The gel holder was securely closed and placed in the half-filled Trans-Blot tank so that the grey panel of the holder was in the cathode side of the tank. The buffer tank was then filled to the bottom of the anode disk before electroblotting at 30V and 400mA, took place overnight at room temperature. Probing the Membrane At the next session, membranes were placed in the staining bath and were covered with ~20mls of Blocking solution for 10 minutes. Then, this was poured away and 5ml of Antibody solution was added to the membrane and it was incubated for 30 minutes at room temperature. The membrane was then washed three times in 10ml TTBS, before adding 5ml of anti-mouse IGG Alkaline Phosphatase Conjugate, and incubated at room temperature for a further 30 minutes. The membrane was washed as before, prior to 10ml of colour development solution was added to the membrane and incubated at room temperature in the dark. After 5-20 minutes, colour development should be complete, and as a result the membrane should be rinsed with distilled water to stop the reaction. The membrane should be placed on filter paper to dry before analysing it. Results SDS-PAGE Following SDS-PAGE, and the removal of the gel, protein standards were identified and the known Molecular Weights (Fig. 1), measured in kilo Daltons (kDa), were calculated and given to the class. The six standard proteins (Table 1) were identified as: Myosin (200 kDa), a motor protein found in eukaryotic tissues, and plays a key role in muscle contraction in the striated and smooth muscle cells along with actin; β-Galactosidase (116 kDa) a hydrolase enzyme, which catalyses the hydrolysis of β-galactosides into monosaccharides and an essential human body enzyme which is also produced in E.coli; Phosphorylase β (97 kDa) a contractile protein found in rabbit muscle; Albumin – Bovine (66 kDa) or Bovine Serum Albumin (BSA) is a serum albumin protein, which has many functions in laboratory techniques, such as ELISAs, Immunoblots and Immunohistochemistry, as well as being commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA; Albumin – Chicken Egg (45 kDa) is a water soluble protein, but the best known form of albumin is egg white; and Carbonic Anhydarse (29 kDa) is an enzyme which catalyses the rapid conversion of CO2 into bicarbonate and protons and is responsible for the carriage of CO2 out of tissues. Table 1 also shows the calculation of MW into log10, based upon kDa weights, and not Da weights. The distance the protein bands travelled on the gel were measured in centimetres (cm) (Table 1), as well as the distance the Unknown protein migrated on the gel, which was 1.1cm. Protein standards measured the following distances: Myosin – 0.4cm; β-Galactosidase – 1.0cm; Phosphorylase β – 1.3cm; Bovine Albumin – 1.8cm; Chicken Albumin – 2.3cm and carbonic Anhydrase – 3.8cm. Figure 1 shows the SDS-PAGE for both the standards and the unknown. The migration of the unknown was found to be similar to β-Galactosidase. Figure 1 allows us to estimate the molecular weight similar to that of β-Galactosidase, at 116 kDa, whereas Graph 1 allows a more quantitative analysis. It shows that the log10 MW of the unknown protein was approximately 2.03. Removing log10, it can be calculated that the MW of the unknown protein was therefore, 107.2 kDa. This MW is very close to that of β-Galactosidase, and therefore, β-Galactosidase must be the Unknown protein. Western Blotting The transfer of proteins from the polyacrylamide gel to the nitrocellulose membrane after SDS- PAGE analysis can be seen in Figure 2. The blue bands seen are the protein bands that have been transferred. The Western Blot involved membrane blocking, in order to reduce non-specific protein interactions between the membrane and the antibody. This was done by placing the membrane in BSA. The membrane was then incubated with the primary antibody, which is specific for the protein of interest, and we now know that that protein was β-Galactosidase. The membrane was rinsed to remove any unbound primary antibody and then incubated with the secondary antibody, which binds to the primary antibody. The secondary antibody is modified in such a way, that it will bring about a reaction and give colour, allowing for visual identification of protein. Figure 2 shows the dark colour production and when analysed against the blue bands, it is clear that the unknown antibody had been identified and therefore the protein had been identified, due to the migration distance, which was similar to β-Galactosidase. TABLE 1: Identified Protein Standards and their Molecular Weights Protein Standards M W (kDa) Log10 MW Distance Migrated (cm) Myosin 200 2.3 0.4 β-Galactosidase 116 2.06 1.0 Phosphorylase β 97 1.99 1.3 Albumin – Bovine 66 1.82 1.8 Albumin – Chicken Egg 45 1.65 2.3 Carbonic Anhydrase 29 1.46 3.8 Table shows the known molecular weight (kDa), calculation of log10 molecular weights of the identified standard proteins (n=6), and the distance protein bands migrated on the gel (cm) which were measured before the Western Blot procedure. FIGURE 1: SDS-PAGE image of Protein Standards and Unknown Protein 200 kDa 116 kDa 66 kDa 97 kDa 45 kDa 29 kDa 1 2 Lane Lane 1 2 SDS-PAGE was run at 200V for approx. 55 minutes as the bromophenol blue band was 1cm away from the bottom of the gel (7.5% Separating Gel and 4% Stacking Gel). Lane 1 shows bands for the Unknown Protein and Lane 2 is the marker lane, showing all bands of the Protein Standards. Molecular Weights (kDa) are highlighted on the side of the arrows. Both samples were incubated in a boiling water bath for 3 minutes, so as to denature the proteins and form the SDS complexes, so that they could migrate onto the gel. The Unknown protein band migrated to a similar point to the second standard protein, at approx. 116 kDa, hence the unknown protein must be β-Galactosidase. GRAPH 1: Log10 MW and Migration Distance of Protein Standards Myosin – 0.4cm β-Galactosidase – 1.0cm Phosphorylase β – 1.3cm Albumin – Bovine – 1.8cm Albumin – Chicken Egg – 2.3cm Carbonic Anhydrase – 3.8cm The graph shows the results of the SDS-PAGE. The chart at the side represents each of the Standards individually. The Log10 MW of Standards is shown against the Distance Migrated on the polyacrylamide gel allowing the MW of the unknown protein to be calculated. The distance travelled of the unknown protein was 1.1cm, therefore, the log10 MW is approximately 2.03. This is very similar to the log10 MW for β-Galactosidase, which is 2.06. Again, indicating that the unknown protein is β-Galactosidase. FIGURE 2: Western Blot Analysis of Standard and Unknown Sample Separated by SDS-PAGE Lane 1 2 The Western Blot was prepared with 5ml of a primary and secondary antibody – 5ml of anti-mouse IGG Alkaline Phosphatase Conjugate. Colour development occurred in approximately 20 minutes. Lane 2 shows the reaction of the second antibody to give a change in colour, and Lane 1 contains the proteins from the gel which transferred on the nitrocellulose membrane, and act as MW markers. Discussion In this experiment, it was concluded that the unknown sample was β- Galactosidase. This protein is a hydrolase enzyme, which catalyses the hydrolysis of β-galactosides into monosaccharides and is an essential human body enzyme. In E. coli, β-galactosidase is produced by activation of the lac operon, as the lacZ gene. The SDS-PAGE technique highlighted the standard proteins very well, making any comparison very easy. Using the SDS-PAGE technique gave more of a definite result, as the MW of the unknown could be measured and read off the graph, whereas in the WB, it was a lot more difficult to define what the colour change represented. Only after several comparisons and careful analysis of the western blot, could it be said that the antibody for the protein, β-galactosidase, was in fact the correct one. Although the correct result was identified, using the two techniques; the experiment could be improved with more practice, and also perhaps doing more than one gel, or western blot. As well as this, the gel could have been run more slowly and carefully, as there were some handling errors. Molecular Weight Of Unknown Protein

Performance appraisal for human resources

Introduction: Usually as everybody thinks that working in human resources has one hard moment. It is hard to recognize when the job is successfully completed. However, human Resources activities and initiatives are usually a long term activities and it is hard to see any result immediately. As we can give a description, for Performance appraisal which may engage too many things such like identification, besides measurement, along with management of individual performance in business enterprises. Business enterprises frequently carry out appraisals designed for managerial, which is a assessment concerning a staff work circumstances, together with promotions along with rewards, also or even developmental, which is a assessment concerning spiraling the staff profession talents, together with counseling along with teaching principles. On the other hand, we can add here that dissatisfaction in the midst of appraisals is widespread. Moreover, on the same subject as it has been disclosed frequently that Human Resource proficient’s, as well line managers, in addition to staff voice frustration. Furthermore, we can find also that a lot of workforces comprise trouble through appraisal that might explanation for the little life extent of the standard appraisal scheme. Etihad Airways Performance appraisal system: Etihad Airways is the National Airline of the United Arab Emirates. The airline was set up and started commercial operations in 2003, in Abu Dhabi, the capital of the UAE. Etihad started with an initial paid-up capital of AED500 million, with the twin objectives of creating an airline that would put up with the UAE flag and expand true Arabian hospitality to their guests. Their name reflects this spirit: ‘Etihad’ is Arabic for ‘united’, and for this reason a representation of the bonding along with the seven emirates that constitute the UAE. So far, Etihad grew at an extraordinary rapidity, accumulation more or less one new route a month. In June 2007, Etihad achieved another milestone: 42 destinations. By 2010, Etihad plan to touch 70 international destinations. The performance appraisal system in the Etihad Airways has a vertical chain of dialogue and reviews must be strengthened, for which the performance appraisal system will play an essential role in the case of Etihad Airways. The prime responsibility for action to preparation failures in performance will lie with supervisors and managers at all levels in the Etihad Airways. Additionally, the Etihad Airways is taking into consideration establishing transparent liability mechanisms and will define the policy framework for exercising responsibilities of both management and staff. Etihad Airways has created and implemented a system that provides tools for employees, supervisors, human resources professionals, and senior leaders to plan, monitor, and evaluate career development opportunities for all employees at and above the director level. In place for many years, such system includes metrics and quarterly reviews of employee ratings, promotions, attrition/hires, developmental opportunities, officer appointments, and assignments to help achieve a diverse workforce. Staff at Etihad Airways motivates supervisors to develop career paths for their employees, including those who may have been who may have been overlooked or underestimated. On staff development, performance management and career support, at Etihad Airways states that the Performance Appraisal System (PAS) has been revised, and a process has been initiated to identify center organizational competencies. Moreover, more systematic approach to mission assignments is being implemented. Analysis Adopting Appraisal: Performance appraisal is a critical activity of Human Resource management. Its goal is to provide an accurate picture of past and or even future employee performance. To achieve this, performance standards are established. The standards are based on the job; related criteria that best determine successful job performance, where possible, actual performance is measured directly and objectively. The primary footstep in the performance appraisal procedure is recognizing the subject that is to be considered. Therefore, it is clear that supervisors should recognize the phases or even aspects of performance, which might find out efficient employment performance. At this point, we can find that such progression looks straightforward, other than it may be rather complex. On the other hand, whether a considerable measurement is lost, staff self-esteem might put up with for the reason of staff that performs fine on such measurement might not be accepted or even satisfied. As we can conclude this subject, whether an unrelated or even minor measurement is incorporated, staff might recognize the complete appraisal progression the same as worthless. The performance appraisal system in Etihad Airways of research has a vertical chain of conversation along with reviews might be strengthened, keen for the same the performance appraisal process will play a vital role in the case of Etihad Airways of research. The prime responsibility for action to preparation failures in practice will depend with supervisors as well as managers at every level in the company of study. Additionally, the Etihad Airways of research is taking into consideration establishing clear accountability mechanisms and will fix the policy framework for exercising responsibilities of both management and staff. Etihad Airways of research has created and implemented a way that gives tools for staffs, besides supervisors, as well human resources professionals, along with a high level leaders to develop, monitor, moreover, evaluate career development chances for every staff at plus above the director level. Furthermore, in order for many years, such plan includes metrics along with quarterly reviews of staff ratings, besides promotions, as well attrition/hires, addition to developmental opportunities, along with officer appointments, also assignments to assist gain a clear labor force. Staff at Etihad Airways of research motivates supervisors to pursue employment manners for their staffs, in addition to those who might have been who can have been overlooked or even underestimated. On staff development, performance management and employment assistance, at Etihad Airways of research states which the Performance Appraisal System has been evaluated, in addition to a case has been initiated to determine center organizational competencies. Moreover, more systematic approach to exercise assignments is being implemented. Conclusion: The new world of work demands employee performance instead of loyalty creativity instead of compliance, and earned rewards instead of entitlements. The role of the Human Resource function is not limited to the policies, practices and systems under its control. That role is important and certainly can contribute to an organization’s success but human resource is in a position to influence the way people are managed across an organization. At the end we can get to say that the Human Resource professional must maintain a thorough understanding of positions and needs across such firm, to help each individual experience the right job fit. Moreover, this should bring into the light yourself with the talents and capabilities of each person in your organization. Keep excellent documentation of testing, job applications, performance appraisals, and performance development plans. While there is some evidence that performance appraisal adjustments have been done because of initiating a performance appraisal, it needs to be emphasized that these studies have looked at particularly specific aspects of the system rather than at the whole system that may have been implemented. Very little research has considered the outcomes of a complete performance appraisal in action. A possible explanation may be the difficulty in evaluating such systems. The evaluation of high performance is principally dependent upon the measurement category selected besides these are in move derived from the underlying thought of performance appraisal. Although, within a given approach to performance appraisal, the execution of specific strategies along with structures might be also similarly than others to provide high performance appraisal, there are no guarantees which this will be the position. Performance appraisal is subject to effect from a difference of factors within not only inside elements like as performance appraisal, structures, processes and evaluation, but also external elements that are far less predictable. High performance appraisal, hence, irrespective of the concept to managing adopted, actually depends upon the alignment of inside systems via the larger system within which the business enterprise is located.