In the story “Bones” by J. Lee Engfer, we meet a young, 27 year old, self diagnosed hypochondriac named Lea who acts as if she doesn’t appreciate life. Lea in the beginning of the story is a pessimist but towards the end we see a change in her ideas and thinking until she becomes optimistic about her life. We also meet a young spirited older woman named Thea who is vivacious and fun loving with a sense of style which makes her almost 1940’s Hollywood-esque, with the neck scarves and painted on eyebrows. The story is a small look into Lea’s life.
We learn key points about her such as her mother passing away, and her self-diagnosed hypochondria. It’s Halloween and Lea and her friend Thea go for a walk to Lake Harriet near Thea’s house. Lea has an epiphany about her life. The theme of the story is about living your life to the fullest. The idea that Lea is a hypochondriac could have an even deeper meaning, such as Lea representing the oppression of society in today which is seen almost everywhere with people who do not make an effort to make their lives worthwhile. Lea’s turning point happens as her and Thea play in one of several piles of leaves.
Perhaps the leaves have a more significant meaning than just leaves? Maybe the dead leaves represent Lea’s dead personality and dull, meaningless life. I believe so. I believe the leaves being played in and stirred about represent a deeper meaning of change and finding meaning in life. A simple action of playing in leaves brought on an epiphany that Lea should start living her life. As they walk back home Lea has to slow down to Thea’s pace, which makes her realize that despite Thea’s age, she is able to fulfill her life with the satisfaction of knowing that if she died today, she would have died alive.
This short story can relate to many things, as it is an issue seen in society very often. Religion can be an example of how it restricts your life from doing certain things, and living in fear of death, when there is nothing to be afraid of. I can also relate this short story to a series of books that I have read written by James Frey. “A Million Little Pieces” and “My Friend Leonard” both follow the story of a recovering drug abuser, author James Frey. James’ addiction led him to believe that his life was worthless, that he was worthless, and he would do anything to end it all, rehab gave him the chance o end those feelings and change his views. His views change when he finds out devastating news that his girlfriend has committed suicide, his outlook and perspective on life completely changes as soon as he’s out of rehab. He becomes optimistic about life, but certain aspects remain pessimistic. A popular piece of writing turned music video written by Mary Schmich called “Advice, like youth, probably just wasted on the young” and it was published in the Chicago Tribune as a column in 1997.
The most popular and well-known form of the essay is the successful music single called “Everybody’s Free to Wear Sunscreen” released in 1999, accredited to Baz Luhrmann (which I highly recommend by the way! ). “Accept certain inalienable truths, prices will rise, politicians will philander, you too will get old, and when you do you’ll fantasize that when you were young prices were reasonable, politicians were noble and children respected their elders” the perfect quote for Lea’s life.
The text gives advice on how to create a worry free life; it is a beautiful piece of writing. I absolutely love how this author uses Irony and Flashbacks. Irony was used when Lea said “It doesn’t affect my functioning in society” while referring to her hypochondria. This statement is false because she underestimates herself, when she can achieve so much more than a stable job, and a feeling of just being “ok”. The way she describes her scattering her mother’s ashes is a beautiful example of a flashback, it is extremely descriptive.
There are however many other techniques used such as personification, metaphors, local color, and symbolism. They all tie together in a beautiful way which makes the reader feel what the character is feeling, and see what the character is seeing. It grasps the character’s emotions and makes the reader understand them. Living your life as if you were scared of death at every corner is a pointless way of living.
People should realize that they need to experience many different things in life to fulfill that void that many people feel when asking themselves “what is the meaning of life”. In my opinion the meaning of life is to live your life without justification form others on why you do something. The point of life is to live, how can you live with a constant fear of death? This short story is an excellent example of a self realization that there is more to life than worrying about every single bad thing that happens. “Live as you will have wished to have lived when
PCR amplification
PCR amplification.
Writing Assignment #10 (maximum score of 10 points) For writing assignment two, we will prepare a single report covering our PCR amplification lab. Be sure to include a bit of information about the source of the MtDNA used in this lab, as well as some information about the ultimate goal of the study (=the aims of the activities involving the PCR product; for us these are: cloning, RFLP, and phylogenetic trees ). Label your sections as follows. ABSTRACT Introduce the major research problem which your study investigates, or what it aims to accomplish (1-2 sentences) Explain the specific aims (or hypotheses and predictions; 1-2 sentences per aim). Explain, in the simplest terms possible, how you tested these aims during lab Provide a summary of the results you obtained (2-4 sentences) Place results in a broader context. What comes next for the project? (1-3 sentences) Example (simulated): Many factors can impact the flow of disease between animals to humans, and thereby influence both public and wildlife health. To efficiently reduce the spread of disease, policy makers therefor need to first understand how these factors act (and interact) within a given ecosystem. Toxoplasma gondii, a protist parasite found in many mammals and birds, can spread to humans via multiple routes including cat feces, and consumption of undercooked meat. Both of these sources can be reduced through public health education. To prioritize informational content in awareness campaigns, it will be useful to learn first what a focal (human) population already knows about the potential sources of disease in this environment. We designed a survey and distributed it among households in the Masai Mara region of Kenya. Our gaol was to determine whether local residents know: 1) that direct removal of cat feces by hand incurs significant disease risk, 2) that this risk is particularly important during pregnancy, when T. gondii can induce neonatal defects, and 3) that undercooked meats can be a source of persistent or lifelong infection by gondii.. ~80% of the surveyed households reported that either “often” or “highly often” semi-feral cats retained for rodent control defecate within food preparation areas of the home. 40% of respondents recognized that it is best to remove this feces using tissues, but only 7% of reported that this is most important during planned or preterm pregnancies. We are currently completing pilot surveys of awareness about food safety during meat preparation. Our findings show that health policies within the Mara region should emphasize risks posed by handling cat feces, especially during pregnancy. Other risk factors still being surveyed, such as meat temperatures, should also be integrated into these plans in accordance with relative awareness levels in surveyed households. INTRODUCTION can include a brief background on the techniques used in the lab, and explanations of the expected outcomes. You may find this structure helpful: 1) A question from the field of study which the experiment targets 2) A list of approaches that can be used to investigate the question? 3) Info about projects in this area that have been completed by other investigators, and also a list of which questions remain unanswered?
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