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Journal is to be at least three (3) double-
spaced, typed pages—but should not exceed six (6) typed pages—and will reflect on and
summarize the unit’s readings. Questions are below. Journal is very similar to papers and therefore are
expected to be well-written and clearly-organized. Journals should be free of typos and other
compositional errors A few hints about references. When you refer to your readings—which I expect from ALL journal submissions—you do not need to provide a formal bibliography. I know what your sources are. HOWEVER, I do expect references in the text (often called “in-text” or parenthetical style). So, when you refer to our textbooks, for instance, use the following format: (Davidson, 32) or (Smith, 104). NO OTHER SOURCES ACCEPTED THE TWO CHAPTERS WILL BE ATTACHED AFTER BID CHAPTERS ARE (50 PAGES )
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Robert Morris University Market Needs and Project Problem Statement Paper.

Look at the attached file. I just want an introduction to this Midterm Progress Report one page From our last week, Mr. Gabe is still working on the task we have given him and then only we would proceed on to which material we have available in our university which we could use depending upon the feasibility of the design and the overall features of the selected material.As for this week, the material deciding task is still on hold so we have decided to focus on other parts of the project. We are currently working on the movement portion of our stabilizing shoulder brace. From the design we have currently developed after careful analysis and study, we are trying to lock the position of the brace so that the user can maneuver as per his/her requirement. The brace would be able to be locked in a horizontal and in a vertical position. The brace would be able to move 180 degrees while in half-circular motion and it can be moved 90 degrees while both moving it horizontally and vertically. A basic sketch is presented in Figure 1 for better understanding (shown on the next page).In Part 1, a feature has been added which allow the user to adjust the tightness of the brace according to the width and length of his/her arm. A flexible and easily moveable tightening screw is added which can be adjusted on the width of the arm as the arm width varies for every human.In Part 2, a locking mechanism has been devised which allows the user to lock the horizontal and vertical position of the brace according to the desire of the user. Apart from easily moving brace, our stabilizing shoulder brace provides an added feature to lock the movement of the brace which can come in very handy while performing certain tasks. Such locking mechanism allows the user to perform certain highly effectively as the brace would restrict movement towards the other direction and thus the task at hand could be performed more easily and with less trouble to keep the brace stable.Finally, Part 3 explains that when the brace is locked in any position, the user then gets a 180 degrees allowance to move the brace according to will.These movements are tentative and are part of the first basic design. Now this design would be implemented physically and then the design feasibility would be calculated. Furthermore, more designs would also be considered and then the most feasible and economic design would be finalized for final prototyping of the stabilizing shoulder brace.
Robert Morris University Market Needs and Project Problem Statement Paper

Extraction of GAPDH Gene from Grass Species. The extraction of the GAPDH gene from grass species, for sequencing, to determine which grasses will be most suitable for the stimulation of hydrocarbon-degradation Introduction: Glyceraldehyde-3-phosphate gehydrogenase gene (GAPDH) is a house keeping gene for a crucial enzyme which catalyses an important step in glycolysis, found in all phylogeny. This gene can be extracted and isolated from plant gDNA through the use of PCR. Once cloned, the GAPDH gene will be sequenced and ultimately analysed through bioinformatics for further research. The grasses that will be investigated throughout this experiment are as follows. Cymbopogon Ambiguous, also commonly known as lemon scented grass, mainly grows in NSW, QLD, NT and WA. This grass is a tufted perennial, which has thin dark green coloured leaves, growing 60-100cm tall approximately. As the common name for this grass states, when this grass is crushed it gives off a distinct lemon smell, allowing it to be easily identified. This grass is also quite a hardy grass, it is very tolerant to wind, drought and frost conditions, allowing it to be grown in a variety of different areas. Themeda Triandra, known as Kangaroo Grass is native to Australia, found to be growing in every state and territory (Unkown, n.d. Native Seeds). This grass is a tufted perennial, growing up to 1.5 m tall and 0.5 m wide. This particular grass is quite coarse, with a grey/green leaves, which produce a very distinct red/brown spikelets (Jennifer Liles, 2004) with the head containing the seeds. Themeda Triandra is considered a good grass for occasional grazing; however it does not tolerate heavy or continuous grazing. Meaning it can be a good indicator for the level of animal activity in a certain grassland area, as if grazed too frequently, the grass has been known to disappear, and not re-grow. Dicantheum Carrisium, known as Queensland Blue Grass is a native Australian Grass, grown not only in Queensland, but all parts of Australia. This Grass was originally very popular in Southern Queensland, due to its optimum performance in the weather conditions of the area. Dicantheum Carrisium is a perennial, with an upright tufted appearance. Growing from 30-80cm tall, and 10-15cm wide with flat broad leaves, It is quite a soft texture with typically blue/dark green colour, making it quite distinguishable from many other grasses. Microlaena Stipoides, known as Weeping Grass, is native to Australia and is found in many of the damper regions from Cape York Town to Tasmania (Unknown, n.d. Native Seeds). Microlaena Stopoides is a slowly spreading perennial, growing to approximately 70cm tall, with weeping seed heads. This is a very tough and tolerant grass, suited to many different pH soil levels <5.5. And a very high tolerance to drought, frost, salt and soil aluminium, although its growth is most abundant in damp shaded areas. This grass has a variety of applications, from grazing to domestic lawns. Once established, Microlaena Stopoides has been seen to be one of the best native grasses used for lawns, because of its tolerable nature, especially its tolerance of deep shade. Pennisetum Alopecuroides, also commonly known as Fountain Grass, is not native to Australia, it is native to Northern and Eastern Africa. But has found its way into Australia, with limited distribution in NSW, but it has naturalised itself in QLD, VIC, SA, WA and NT. Pennisteum Alopercuroides grows in a mound of foliage, growing to 10mm wide, and up to 150 cm tall. (Jim Brockmeyer, 2014). This grass type contains bottle-brush like seedheads, which are on stems reaching to the tallest point of the plant. Due to these seed heads, the plants seed can be distributed in a variety of directions very easily by wind. Allowing for the plant to spread quickly and easily. It is a very tough and hardy species. Poa Iabilardieri, also known as Tussock Grass is native to Australia and found mostly in the south-eastern regions of Australia (Unknown, n.d. Native Seeds). It is a thick perennial grass with bright blue/Green soft weeping foliage, which grows to approximately 45cm tall, and 45cm wide. Poa Iabilardrdieri is tolerant to being exposed to full sun, but grows best in damp areas. These Australian Grasses can be used in a variety of ways, some being investigated for their suitability for Bioremediation. Bioremediation is the use of biological organisms to treat soils containing organic chemicals (Government of SA, 2005). This technology is an environmentally sound, and cost-effective way of potentially eliminating contaminants in soils. Gaskin (2010) investigated into Australian grasses and their individual ability to stimulate the removal of aliphatic hydrocarbons from a mine site soil (Shayrn E. Gaskin, 2010). In this study three species were further investigated, two which are included in our study, Cymbopogon Ambiguous (Lemon Scented Grass), and Microlaena Stipoides (Weeping Grass). It was found in this study, that both C. Ambiguous and M. Stipoides stimulated the hydrocarbon-degredation the most. However the way in which these plants were stimulating this effect was unknown. The aim of this experiment is to obtain a sequence of GAPDH gene from one of the 6 grass species discussed, and determine their suitability for bioremediation. This will be achieved through using PCR on the extracted gDNA from the grass sample. Once the initial PCR has been done, a second set of primers is used to amplify the PCR products, this technique is called nested PCR. Once the PCR products have been cleaned, using size exclusion column chromatography; separating the DNA from the proteins, primers and nucleotides, a ligation is performed on the DNA, and Bacterial E.coli cells will be used for transformation. The transformed colonies will be inoculated into miniprep LB ampicillin to be cultured (Flinders University, 2014), and the Bacteria is grown. Once this is done, the plasmid DNA will be purified , and cut with Not I, allowing for it to be prepared for sequencing, and analysis. Once sequenced it may be possible to see a difference between the grasses GAPDH gene, which may indicate as to which Australian grasses will be effective when it comes to bioremediation. Words: 978 Reference List: Government of SA. (2005).Soil bioremediation [online] .Available at: http://www.epa.sa.gov.au/xstd_files/Site contamination/Guideline/guide_soil.pdf. Last accessed 3rd April 2014 Jennifer Liles. (2004).Themeda triandra.Available at: http://www.anbg.gov.au/gnp/interns-2004/themeda-triandra.html. Last accessed 6th April 2014. Jim Brockmeyer. (2014).Pennisetum alopecuroides – Fountain Grass.Available at: http://www.bluestem.ca/pennisetum-alopec.htm. Last accessed 9th April 2014. Sharyn Gaskin, Kathleen Soole, Richard Bentham. (2008). Screening of Ausralian Native Grasses For Rhizoremediation of Aliphatic Hydrocarbon-contaminated soil. In: ZInternation Journal of Phytoremediation. Adelaide: ZZ. 378-386 Sharyn E. Gaskin, Richard H. Bentham. (2010). Rhizoremediation of Hydrocarbon contaminated soil using Australian Native Grasses. In: Science of the Total Environment. Adelaide: 3683-3684. Unknown. (n.d.).Kangaroo Grass.Available at: http://nativeseeds.com.au/Kangaroo-Grass.php. Last accessed 10th April 2014. Unknown. (2013).Lemon-Scented Grass Profile.Available at: http://www.environment.nsw.gov.au/threatenedspeciesapp/profile.aspx?id=10267. Last accessed 7th April 2014. Unknown. (2014). Planning Guide. In: UnknownGeneral Information and Laboratory Manual. Flinders University: Flinders University. 38-55. Unknown. (n.d.).Weeping Grass.Available at: http://nativeseeds.com.au/Weeping-Grass,-Microlaena-Stipoides.php#sthash.Llv576Vn.dpuf. Last accessed 10th April 2014 Unknown. (n.d.).Tussock Grass.Available at: http://nativeseeds.com.au/Tussock-Grass.php. Last accessed 9th April 2014 Bianca Schmidt Extraction of GAPDH Gene from Grass Species

Diverse Workforce, PowerPoint Assignment Homework Help

Diverse Workforce, PowerPoint Assignment Homework Help.

Imagine that you work for a company with an age diverse workforce. You have baby boomers working with millenials. Their backgrounds are different, and how they view work is different. This is causing some friction within the workforce. Before the tension escalates, you need to have a meeting to discuss the issue. Prepare a five to seven (5-7) slide PowerPoint presentation for your staff meeting that addresses this issue and proposes a solution.Create a five to seven (5-7) slide PowerPoint presentation in which you:Propose a solution that will relieve friction in your company’s age diverse workforce.Format your assignment according to the following formatting requirements: Format the PowerPoint presentation with headings on each slide and at least one (1) relevant graphic (photograph, graph, clip art, etc.). Ensure that the presentation is visually appealing and readable from up to 18 feet away. Check with your professor for any additional instructions.Include a title slide containing the title of the assignment, your name, your professor’s name, the course title, and the date.The specific course learning outcomes associated with this assignment are:Explain effective approaches to the broad spectrum of employee relations, including career development, fostering ethical behavior, discipline, labor relations, and dismissals.Use technology and information resources to research issues in human resource management.Write clearly and concisely about human resource management using proper writing mechanics.
Diverse Workforce, PowerPoint Assignment Homework Help

Vocabulary Game Adapted Lesson Plan Worksheet

python assignment help Vocabulary Game Adapted Lesson Plan Worksheet.

Part 1 Create an engaging vocabulary game to be used to teach key vocabulary words to ELLs in your selected content area. Your vocabulary words should address Tier One, Tier Two, and Three Tier words from your required reading which include: Common words, which most children acquire in daily conversations in their first language; Content specific technical words, which may be commonly used or rarely used; and Words that define written text which are generally more sophisticated and precise than words used in conversation or academic vocabulary from academic texts. Your submission should include: A list of all of the Tier One, Tier Two and Tier Three key vocabulary words. A detailed description of the game rules, number of players, and how the game is played. The students’ grade level, core content area, and ELP level on your submission documentPart 2 Complete the worksheet provided
Vocabulary Game Adapted Lesson Plan Worksheet

High Turnover Rate in Long-Term Care in Nursing

Lisa Krier Introduction There is a serious problem in this nation, and it is only getting worse. By the year 2030, the number of elderly in the United States, 85 years or older, is expected to reach 8.9 million (Barondess, 2007). According to the Center for Disease Control and Prevention’s website, two-thirds of people reaching the age of 65 will require the services of a long term care facility at least once during their lifetime (Harris-Kojetin, Sengupta, Park-Lee, Valverde, 2013). As the population continues to age, the population of those over the age of 65 is projected to soar to 88.5 million by the year 2050, and the oldest of the old, those 85 years and older, is estimated to reach 17.9 million and account for 4.5% of the U.S. population (Harris-Kojetin, Sengupta, Park-Lee, Valverde, 2013). The 85 and older population often have the highest disability rate and their need for long term care placement is the greatest (Harris-Kojetin, Sengupta, Park-Lee, Valverde, 2013). Unfortunately, the number of women who are 20 to 50 years old, the population most likely to provide the work force of direct care staff, is only expected to increase less than 10% during this same time (Barondess, 2007). In the United States, the shortage of direct-care staff is a serious problem that is worsening (Barondess, 2007). Recruitment and retention of direct- care staff is extremely challenging and is exacerbated by the growing division between the number of those needing care the number of those providing the care (Barondess, 2007). Discussion Long term care facilities experience very high turnover rates among direct-care staff (Barondess, 2007). This problem is costly, threatens the quality of care provided to patients, increases workloads, and can lower morale among the remaining direct-care staff, with all of this contributing to continual and increased turnover (Barondess, 2007). According to the Institute of Medicine’s website, among direct-care staff there was a 71% turnover rate nationwide in 2008 and they were more likely to not have health insurance and to use food stamps (IOM, 2008). The high turnover rate of direct-care staff costs employers on average $4.1 billion annually (IOM, 2008). Direct-care staff contributes greatly to the physical and mental health of long term care patients (Stone

The nature of health promotion work in midwifery

Health promotion is an essential part of a midwives responsibility; “the nature of health promotion work in midwifery is geared toward promoting the health of the mother and ensuring an optimum environment for mother and baby” (Dunkley, 2000:40). Breastfeeding can be a controversial topic. It can bring about mixed opinions and responses from mothers and midwives. In spite of the message “breast is best” bottle feeding has become part of the culture in Ireland’s society. “Health promotion is predominately a proactive process. It is a process that is done with people not at people, either on an individual basis or within groups. Participation and partnership are key components of the process (Dunkley, 2000:42). This essay will discuss the unique ability of midwives in their contribution to the health promotion of breastfeeding in Ireland. A general role of midwives is to enthusiastically support and advise a breastfeeding woman. A midwife should help the woman recognise that breastfeeding is a normal life event in every culture. In order to do this a midwife should have a broad knowledge of the anatomy of the breast and sufficient clinical skills. This will ensure the woman receives adequate information and skills on the postnatal ward, or antenatally. Benefits of Breastfeeding Firstly the reason for the promotion in regard to breastfeeding is that apart from being economically friendly, it also holds many benefits for the baby and for the mother herself. “Extensive research using improved epidemiological methods and modern laboratory techniques documents diverse and compelling advantages for infants, mothers, families and society from breastfeeding and the use of human milk”. (Chalmers