Description: You will submit a 4-page paper based on a non-US country -ITALY-. Students will discuss a supply and demand dilemma and how it impacts the economic climate, as well as two of the topics from the following list: real GDP per capita, unemployment, inflation, and the national debt. Students will also state the relevancy of the research as a tourist, future employee/employer in that nation, or concerned global citizen. Specifications:Introduction: Basic background about the county (location, population, what the country is known for) and why you chose that country. This segment should not be more than 8 sentences.Supply and demand dilemma (using the applications from Chapter 3): find an article that expounds on a factor or factors that shift the supply and/or demand curves within a particular market for your country. Discuss how that factor(s) impacts the demand/supply curves and overall price and quantity. Ensure that you address a particular market or industry and not an aggregate demand/supply (Chapter 12) focus. Economic trends: From the options below, select GDP and another trend to discuss in detail. Ensure that you do not define the terms that you discuss, but that you focus on the changing trends (using the appropriate terminology) and the cause(s) of the changes. This section should be at least 1.5 pages.Real GDP per capita (required)– any major significant changes /trends within the past 3 years and discuss what is causing the changes (hint: think of what is changing in consumption, investment, government purchases, and net exports). *Unemployment rate (option #1)- any changes/trends in the past 3 years (good/bad) and discuss what administrators are doing to improve and/or maintain values. In your analysis, also include a brief discussion about what demographics of people suffer from higher (or lower) levels of unemployment and why.*Inflation (or CPI if you are unable to access the information on inflation) (option #2) – any significant trends or major changes in the past 3 years and at least two factors that are causing the changes. *National debt (option #3) – Review the last 3 years of national debt information. Is the country spending within its means? Explain what is causing the governing branch to stay within the countries’ financial means or why the governing branch is not able to stay within its financial means.*Discuss why this is important for you to be informed about the economic climate of this particular country with regards to being a tourist, future employee/employer or concerned global citizen. This segment should be no more than 7 sentences.Conclusion: Based upon the analysis where do you think the country stands and how can that country improve its economic status? This segment should be no more than 8 sentences.*- Graphs are required for the macroeconomics indicators. Graphs should reflect a minimum of the last 3 years.Other project specifications:Project paper must be 4 complete pages (the fourth page should be filled with the paper content) (double-spaced) Times New Roman 12-point font. The page set-up must be 1-inch margins. Graphs, charts, cover pages, and reference pages will not count toward the 4 pages that are required. The graphs and charts (for each economic indicator, which is a requirement for the project) must be on a separate page after the body of the paper and properly cited. This is a website that gives some examples of how the information should be displayed (properly citing the graphs):https://www.lib.sfu.ca/help/cite-write/citation-style-guides/apa/tables-figures Those specific indicators that require a graph/chart have an asterisk beside it in the specifications portion of the project. All graphs should be downloaded from an economic data resource page and properly cited.Papers should have at least 7 reputable sources. Please note that Wikipedia is not a reliable source for research papers.The paper must use APA documentation (in-text and reference page required). No plagiarism will be tolerated.See website for more details: https://owl.purdue.edu/owl/research_and_citation/apa_style/apa_formatting_and_style_guide/general_format.htmlPlease note that if the project specifications are not met, points will be deducted as deemed appropriate by the instructor.Useful websites for research:Federal Reserve Bank of St. Louis: Fed LinkThe World Bank: World Bank LinkInternational Monetary Fund: IMF LinkFederal Reserve Bank of New York: Fed of NY Link
Midlands Technical College Italy Supply and Demand Dilemma Discussion
I’m working on a communications writing question and need a sample draft to help me learn.
Write 3 paragraphs answer any of these questions:When you are giving speech or talk, what are the ideal nonverbal communication elements you would like to receive from your audience or classmates? What would that look like? Do you believe you reciprocate the same nonverbal ideals to others when they are speaking or communicating? When you are involved in an intimate conversation with people, what are your nonverbal pet peeves from them? What feedback have others given you about your nonverbal support? Are you regularly consistent with your nonverbal and verbal communication? Briefly explain.Sometimes we do not know our “internal” rules about space and touch until they are violated in some way. Can you think of a time when you felt that someone violated a personal space or touch rule? What happened? What was the identity of this person (i.e., were they in a position of power or dominance, such as a supervisor)? How did you handle the situation? Did you handle it nonverbally? Did you say anything to this person? Did you adjust to the situation? Finally, what are some nonverbal communication elements you would like to improve upon? If you would like to improve upon gestures, in what context and how will you know you are successful? If you would like to work on eye contact, how will you know you are successful?
Nonverbal Communication Essay
Purification of Mitochondria by Subcellular Fractionation of Mouse Brain
Purification of Mitochondria by Subcellular Fractionation of Mouse Brain. This paper explores the hypothesis that there are varying quantities of mitochondria between mutant and healthy organisms. The experimental aim of this investigation was to create a method to obtain a subcellular fraction that was enriched in mitochondria. We initially designed an experiment for the purification of mitochondria from mouse brains using subcellular fractionation. Using differential centrifugation and marker enzymes (succinate dehydrogenase) we were able to obtain a subcellular fraction that was enriched in mitochondria. We compared the results from control and mutant mouse brains, through the analysis of succinate dehydrogenase, we found that Pellet 2 (P2) had the highest specific SDH activity that that there was no significant difference between the control and mutant except from the SDH activity of the Homogenate (H) and Pellet 2 (P2). From the data we had obtained we concluded that Pellet 2 (P2) is where all the SDH was, therefore the mutation doesn’t affect the protein content, but it does affect the SDH activity. Most eukaryotic cells contain mitochondria, which is a membrane enclosed organelle that creates most of the cells energy in the form of Adenosine triphosphate (ATP). The inner mitochondrial membrane contains the electron transport chain. The electron transport chain is considered the molecular apparatus for energy production. The Electron transport chain consist of five protein complexes. In the chain, complex I, III and IV pump protons from the mitochondrial matrix to the intermembrane space. This generates a proton gradient which is essential for the synthesis of Adenosine triphosphate. Complex V is commonly referred to as ATP synthase, it acts as the final enzyme in the production of ATP via oxidative phosphorylation. ATP synthase uses the energy resulting from the proton gradient to drive the synthesis of Adenosine diphosphate and Phosphate to form Adenosine triphosphate. Additional functions of the mitochondria include programmed cell death (apoptosis), calcium signalling and cellular differentiation. Neuronal energy supplies are completely dependent on mitochondrial oxidative phosphorylation. Neurons have limited capacity to obtain energy through glycolysis when oxidative phosphorylation is compromised (Herrero-Mendez A, 2009) which makes them particularly vulnerable to mitochondrial dysfunction consequently it is a vital component of brain functioning. To understand mitochondria in the brain it is necessary to purify mitochondria and carry out enzyme activity assays. We decided to use Sub cellular fractionation to isolate the mitochondria in the mouse brain tissue. this is a process used to separate the cellular components of a cell whilst preserving the individual components of each cell (Alberts B, 2002), the process allows each component of the cell to function normally thus preserving the integrity of the components of the cell. We used sub cellular fractionation alongside differential centrifugation to purify the mitochondria so we could carry out enzyme activity assays. The components of a cell were separated based on their density in a centrifuge according to the centrifugal force they experience . The processes combined provided us with an enriched source of protein that has been purified. The fractions we obtained were assayed for protein using Bradfords reagent and Succinate Dehydrogenase (SDH) activity. Succinate Dehydrogenase is only present in mitochondria therefore the distribution of SDH will indicate the distribution of mitochondria in the fractions. Six mouse brains were collected (three control and three mutant) and weighed using a bench top balance. Ice cold Buffer A (20 mL of 0.25 M sucrose, 10 mL of 5 mM Tris-HCl pH 7.5, 3 mL of 3 mM KCl and 0.6 mL of 0.3 mM EDTA) was added to each mouse brain (1:10 (w/v). we homogenised the mixture with 20 strokes of a Dounce homogeniser. 500 µL of the Homogenate was collected and kept aside for further assay (H). The rest of the Homogenate was transferred into two centrifuge tubes and centrifuged at 2,700 rpm for 10 minutes in a 10 x 20 mL MSE rotor at 4 oC. The supernatant was transferred into two clean centrifuge tubes, and the pellet (P1) was resuspended in 5mL of Buffer A. The Supernatant was then centrifuged at 9,300 rpm for 20 minutes in a 10 x 20 mL MSE rotor at 4 oC. the supernatant was collected (S) and the pellet (P2) was resuspended in 5mL of Buffer A. Fractions (H), (P1), (P2) and (S) were assayed using Bradfords reagent (0.004% (w/v) Comassie Brilliant Blue G-250, 4.2% (v/v) phosphoric acid and 2.1% (v/v) ethanol.). The fractions were diluted as the following: 100 µL of homogenate (H) was diluted in the ratio of 1:50 with Buffer A, 200 µL of P1 was diluted in the ratio 1:20 with Buffer A, 200 µL of P2 was diluted in the ratio 1:50 with Buffer A and the supernatant (S) was not diluted. 50 µL of each fraction was transferred into a duplicate and 200 µL Bradford reagent was added. The diluted fractions were incubated for 5 minutes and then read at 650 nm in a spectrophotometer. Fractions (H), (P1), (P2) and (S) were assayed using Succinate dehydrogenase (Sodium succinate (50 mM), Potassium phosphate buffer (pH 7.4, 65 mM), 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium (INT) (50 mM). The fractions were diluted as the following: Homogenate (H) was diluted in the ratio 1:10 with Buffer A, (P1) was diluted in the ratio of 1:10 with Buffer A, (P2) was diluted in the ratio of 1:20 with Buffer A, Supernatant (S) was not diluted. 20 µL of each fraction was transferred into duplicates with 130 µL phosphate buffer, 50 µL INT and 50 µL sodium succinate for 15 min in a 30 oC water bath. The absorbance was read at 490 nm in a spectrophotometer. Figure 1. total protein content in the homogenate (H) and each fraction (P1, P2, S) for control and mutant mouse brains. Average total protein (mg) was higher in the Homogenate (H) compared to Pellet 1 (P1), Pellet 2 (P2) and S. The decrease of average total protein between the homogenate and pellet 1 and S was not significantly different (p ≥ .05). However, the decrease of average total protein between homogenate and pellet 2 was significantly different (p ≤ .05 ). The total protein content was measured using a Bradfords assay. Error bars represent SEM of n = 3 mice. Figure 2. % recovery of protein in each fraction and the homogenate for control and mutant mouse brains. Average % recovery of protein was highest in the homogenate and decreased considerably in each fraction. The difference in % recovery between homogenate and pellet 1 (P1) and (S) was not significantly different (p ≥ .05) suggesting the difference was due to chance. However, the difference in average % recovery in protein between homogenate and pellet 2 was significant (p ≤ .05) suggesting that the difference is not likely to be due to chance. Error bars represent SEM of n = 3 mice. Figure 3. specific activity of SDH in the homogenate and each fraction for control and mutant mouse brains. The specific SDH activity was highest in the homogenate (H) and pellet 2 (P2) and was considerably lower in pellet 1 (P1) and (S). The difference in SDH activity between homogenate (H) and pellet 2 (P2) was significantly different (p ≤ .05) suggesting that is it not likely to be a result of chance. However, the difference in SDH activity between homogenate (H) and Pellet 1 (P1) and (S) was not significantly different (p ≥ .05). The SDH activity was measured using an SDH assay. Error bars represent SEM of n = 3 mice. From the data we obtained we found that Pellet 2 (P2) had the highest specific SDH activity and there was a significant difference in SDH activity between homogenate (H) and pellet 2 (P2), this led us to conclude that pellet 2 (P2) is where all the SDH was, therefore suggesting that the mutation doesn’t affect the protein content but it does affect the SDH activity. It is for this reason we believe that we have obtained a fraction enriched in mitochondria (pellet 2). References Alberts, B; Johnson, A. “Fractionation of Cells”. Molecular Biology of the Cell. 4th edition, 2002. Herrero-Mendez A, Almeida A, Fernandez E, Maestre C, Moncada S, Bolanos JP. The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C-Cdh1. Nat Cell Biol. 2009;11:747-52. Purification of Mitochondria by Subcellular Fractionation of Mouse Brain
SBU Five Perspectives of Time as It Relates to Buying Customers Shop Discussion
essay help online free SBU Five Perspectives of Time as It Relates to Buying Customers Shop Discussion.
I’m working on a marketing question and need guidance to help me study.
InstructionsThe textbook refers to five perspectives on time: Pressure cookerMapMirrorRiverFeastPlease give an example of each viewpoint and describe the situation using a product or service.The learning outcomes associated with this assignment include application of the psychological and neurological foundations that influence consumer behavior, analyzing specific models of consumer behavior and purchase decision frameworks that inform marketing strategies and interpreting consumer segmentation, brand equity, loyalty, brand extendibility, brand personality and visualization in attracting consumers.In a real-world work situation, this assignment will enable students to assess a brand’s custom journey through the lens of time spent on their journey and develop strategies that are most effective given the preponderance of customer types in their audience.
SBU Five Perspectives of Time as It Relates to Buying Customers Shop Discussion
American InterContinental University Unit 4 Honolulu Police Department Offenses PPT
American InterContinental University Unit 4 Honolulu Police Department Offenses PPT.
In Unit 4, you practiced analyzing statistical data. In this activity, you will further practice your analytical skills by creating 5 different charts from the data you analyzed in Unit 4. Additionally, you will create a presentation including each of those charts and explain why you used the selected chart.
The specific steps are as follows:
Download 1 of the following datasets of offenses reported:
Accomack County Sheriff’s Office
Honolulu Police Department
Los Angeles Police Department
Using the provided data and your analyzed data, create 5 different charts.
Create a PowerPoint presentation effectively presenting each of your charts, with a written description of each chart.
American InterContinental University Unit 4 Honolulu Police Department Offenses PPT
List three examples of plagiarism and discuss how plagiarizing as a student affects the integrity of a baccalaureate degree, the
List three examples of plagiarism and discuss how plagiarizing as a student affects the integrity of a baccalaureate degree, the public perception of the nursing profession, and evidence-based practice. Describe two things you will do to ensure academic integrity in your work. FYI: Using 200-300 words APA format with at least two references. Sources must be published within the last 5 years. There should be a mix between research and your reflections. Add critical thinking in the posts along with research. Apply the material in a substantial way.