For this project you will need to apply simple linear regression and study a problem of interest. In this project, you will perform a statistical analysis to investigate how two quantitative variables (not qualitative variables) are associated and how one influences the other. You can choose what two variables you are interested in studying and will collect your own data in order to perform an analysis. I will provide you with a few good sources from which you might be interested in collecting your data. The requirements for this project are discussed in detail below.Use of Excel or other computer statistical software will be required to carry out various calculations, produce tables and graphs, and to perform a statistical analysis. You will be required to write a 2.5 to 3 page report (double-spaced) briefly discussing the problem being studied, your analysis and findings, as well as additional or concluding remarks. Note that the 2 to 3 page minimum length does not include any tables or graphs (which should be included separately with the paper). You will need to provide the Excel graphs or charts produced along with the report, as well as the raw data collected.The report section of the project needs to be well-written with good sentence structure and proper grammar. You must include an introduction and a strong conclusion, as outlined in the requirements below:A. Requirements for the Written Report (Minimum 2.5-3 pages, double-spaced)a) An introduction paragraph discussing the problem being studied, some background on the topic, and why it is of interest to you. Mention how your data was obtained and cite your source.b) Describe somewhere which variable would be the explanatory variable and which one is the response variable, and why so.c) Interpret the meaning of the correlation coefficient in context of the problem and what this means.d) Include the linear regression equation in the report with the determined intercept and slope values. Also, interpret the slope and intercept values. Lastly, for a particular chosen value of the explanatory variable predict what the response variable would be and interpret what this would mean in a sentence. (Make sure the explanatory variable value chosen would make sense for this problem)e) Find what the coefficient of determination value is, and interpret it in the context of this problem being studied. Does this indicate that the model is a good fit for the data and why?f) Discuss, based on your findings, if there is a significant linear relationship between the two variables.g) Perform diagnostics on the regression model using residual plots. Using these, comment on whether or not a linear model should be appropriate, on whether or not the residual error term appears to have constant variance, and on whether or not there are any outliers.h) Briefly describe, in conclusion, if this regression model does a good job in explaining the dataset, based on your Excel findings. Here you can also make additional comments about the regression model that you think are worth mentioning. This is your chance to be creative and provide additional insight.B. Requirements for the Appendix After the Written Report:a) A scatter diagram showing the relationship between the 2 variables being analyzed. Include a graph of the linear regression equation in this plot as well. Be sure to label the axes and the plot.b) Show the tables, determined using Excel toolbars and functions, which display coefficient values, t values for the regression coefficients, and the p-values.c) The residual plot, as shown in class. Be sure to label the axes and the plot.d) The raw data collected.e) Describe how you and your group member each contributed to the project.Please no plagiarism, as it will be checked when submitted.Thank you!
Los Angeles Pierce College Blood Fat Simple Linear Regression Project
Read the Article below and write a two paragraph reflection. What questions does it raise to you? Be prepared to discuss it in class. Copy and paste your reflection in the submission box on Canvas._________________________________________In one of the most provocative and misunderstood studies of the year, scientists in the U.S. and Switzerland have made an astonishing discovery: All humans alive today are the offspring of a common father and mother – an Adam and Eve – who walked the planet 100,000 to 200,000 years ago, which by evolutionary standards is like yesterday. Moreover, the same is true of nine out of every 10 animal species, meaning that nearly all of Earth’s creatures living today sprang into being recently from some seminal, Big Bang-like event.Mark Stoeckle at Rockefeller University and David Thaler at the University of Basel reached this striking conclusion after analyzing the DNA “bar codes” of five million animals from 100,000 different species. The bar codes are snippets of DNA that reside outside the nuclei of living cells – so-called mitochondrial DNA, which mothers pass down from generation to generation.With each reproduction, errors creep into the bar code, as they do when you repeatedly photocopy a document. By measuring the accumulated errors – the blurriness or “diversity” among the bar codes –scientists are able to infer the passage of time.That’s how Stoeckle and Thaler concluded that ninety percent of all animal species alive today come from parents that all began giving birth at roughly the same time, less than a quarter-million years ago. “This conclusion is very surprising,” Thaler avers, “and I fought against it as hard as I could.”What caused animal life on Earth to be almost completely renewed such a short time ago? For now, it remains a mystery.It’s possible something far more powerful than H-bombs decimated life and only a single set of parents for each species survived to live and procreate another day. But the last major extinction event we know about – the one that snuffed out the mighty dinosaurs – happened a full 65 million years ago.It’s also possible there is something in nature that limits the size of an animal population. Perhaps it’s some built-in evolutionary process that when a population gets too big, it crashes and must restart itself from scratch.In their report, published in Human Evolution, Stoeckle and Thaler offer other possible explanations, including, Thaler explains, “ice ages and other forms of environmental change, infections, predation, competition from other species and for limited resources, and interactions among these forces.” Whatever the explanation, he adds, the takeaway is this: “all of animal life experiences pulses of growth and stasis or near extinction on similar time scales.”That is, humans, elephants, birds, you name it – Earth’s creatures tend to stand and fall in unison, like the rising and falling of the tides. And even though we don’t know what Svengali is directing the show, we now have scientific evidence that it wipes the slate clean far more frequently than we ever imagined.Many religious commentators misunderstand this study to mean that species abruptly came into being only recently. To be clear: according to evolutionary biologists, species developed gradually over many millions of years. Stoeckle and Thaler’s discovery is that something happened roughly 100,000 years ago that created entirely new populations from long-existing species.That said, Stoeckle and Thaler’s study does line up with the Bible in at least two notable ways. First, it affirms that we and our fellow creatures on Earth arose from a recent and profound creation event, orchestrated by some unknown mechanism. And second, the DNA bar codes reveal that species are quantized. Instead of there being a continuum of animal varieties, as one might expect from millions of years of gradual evolution, creatures fall into very distinct, widely separated populations – what the Bible describes as “kinds,” from the Hebrew word min.“Darwin struggled to understand the absence of intermediates and his questions remain fruitful [to this day],” says Thaler. What the new study discovered, he explains, is that “If individuals are stars, then species are galaxies. They are compact clusters in the vastness of empty sequence space.”Above all, Stoeckle and Thaler’s intriguing study serves up hard evidence for the astonishing fragility of life and a humbling lesson for the high-and-mighty among us. It proves we are at the mercy of forces we do not see nor understand; forces that made our existence possible a few hundred thousand years ago and that just as quickly – in the twinkling of an eye – can and will one day take us out.
PHI 1600 Barry University Extinction Event Precede Adam and Eve Reflective Paper
FMS 191 University of California Inspiration and Perception of a Movie Worksheet
FMS 191 University of California Inspiration and Perception of a Movie Worksheet.
I’m working on a film project and need a sample draft to help me understand better.
Having looked at a lot of good examples for title sequences, you are now going to imagine
pitching an idea for producing your own – at least you will storyboard one. Please develop
your idea – either for the main titles of a new soap opera about UC Irvine, or else
those of a show about UC Irvine sports and Anteaters fans.
Before storyboarding your sequence, you must decide on your target audience, when
and where your imaginary program will be scheduled, its title, setting, protagonists, stock
characters, some suggested plot lines, and themes. Your storyboarded title sequence must be
no longer than 45 seconds, and needs to convey the genre of your program. You need to
include a sense of important locations and settings, an introduction to important characters,
and an idea of key themes, concerns, or storylines of the program.
Please study the very detailed storyboarding handout – it gives valuable advice on
camera angles, length of shots, sound effects and/or music, and any other elements
and effects you may use. You should also think about fonts, color, style, and lighting.
When you storyboard your concept for the main titles of this imaginary new series, bear
in mind that all elements should work together to convey the genre, playing into (or perhaps
playing with) conventions and codes that allow you to signal who the target audience is and how
the main titles want the series to be understood.
In grading this homework assignment, I am interested generally in the strengths of your
storyboarding detail, but above all in how successfully the content and form of your
title design idea pitches a title sequence for your target audience. I expect that you will need
at least 10-15 (ten-fifteen) frames to storyboard your title sequence of (up to) 45 seconds.
Whatever storyboarding grid you use, make sure to cover the following required information –
STUDENT ID # AND NAME
protagonists, stock characters, suggested plot
themes, typographic and other design elements, music and/or sound design
FMS 191 University of California Inspiration and Perception of a Movie Worksheet
ECON 3307 Saint Marys University Special Purchase and Resale Agreements Questions
online dissertation writing ECON 3307 Saint Marys University Special Purchase and Resale Agreements Questions.
I’m working on a business test / quiz prep and need an explanation to help me learn.
Analyze each of the following statements and determine whether it is True, False, or Uncertain. Marking will be based entirely on the supporting arguments. Therefore, try to be thorough in your answers and base your discussion on the theories that have been reviewed in this course.a.The effect of an open market purchase on reserves differs depending on how the seller of the bonds keeps the proceeds. If the proceeds are kept in currency, the open market purchase has no effect on reserves; if the proceeds are kept as deposits, reserves increase by the amount of the open market purchase.b.If the overnight rate is below its target for the day, the Bank of Canada will enter into Special Purchase and Resale Agreements (SRPAs) to drive it up to its target.c.If Canadians refrained from using cash and started using credit cards instead, the money supply will increase, other things being equal.d.In the money creation process the simple money multiplier ignores the possibility of currency drain. That resulted in the monetary base being overstated. e.Investors will probably wish to buy bonds when interest rates are low in the hope of selling them at higher prices when interest rates high
ECON 3307 Saint Marys University Special Purchase and Resale Agreements Questions
Florida Southwestern State College Mod 5 Gender Stereotypes and Roles Questions
Florida Southwestern State College Mod 5 Gender Stereotypes and Roles Questions.
I’m working on a gender studies question and need a sample draft to help me learn.
Module 5: Chapter 5 Sexual PhilosophyDue Sunday by 11:59pm Points 35 Submitting a file upload Overview & Guidelines Remember, when writing your sexual philosophy step into the mindset of a philosopher, and engage in the pursuit of wisdom, search for an understanding of your values, and analyze your beliefs, reasoning, and attitudes. The goal of establishing your sexual philosophy is to be inquisitive and explore how your thoughts and behaviors influence the quality of your personal life, relationships, society and the world at large.Part 1:At the very end of the chapter (past the Summary section) locate the section titled: Questions for Discussion. Select and respond to ONE of the Questions for Discussion (Note: If you would prefer to write your sexual philosophy on something else discussed in the chapter instead of one of the Questions for Discussion, feel free to do so). Your response should be a minimum of 100 words, and your word count should be indicated at the end of your statement.”Questions for Discussion:-“How have gender stereotypes and roles influenced your views of your sexuality and the ways in which you relate to others-If you had an infant born with ambiguous genitalia, would you opt for surgery? Inhibit the onset of puberty with drugs? What gender would you raise the child? If surgery were chosen, when the child was old enough, would you inform him or her about this treatment? Or would you not choose surgery and instead leave the decision to the individual at a later time?-Do you believe that your gender identity was biologically or socially determined? Who or what most influenced your gender identity? In what ways?”Part 2:At the very end of the chapter (past the Questions for Discussion section) locate the section/box titled: Sex and the Internet. Follow the instructions in this box making sure to clearly respond to all of the prompts.”Sex and the InternetWorking for LGBTQ Equal RightsThe number of education and advocacy groups working around sexual orientation and gender has increased tremendously in recent years. Go to the Human Rights Campaign (http://www.hrc.org). From there, click “Topics,” identify two topics of interest, and read what they have to offer. Once you have read about two issues, answer the following questions:-How does the new information you have gathered influence the way you think about gender and/or sexual orientation?-What was one specific aspect of this subject that most interested you?-What is one point you still have questions about?-What have you learned as a result of this research?”Part 3:Select ONE of the “Think About It” sections in this chapter and provide a thorough response to the corresponding Think Critically questions. “Think Critically-Have you or someone you cared about been hassled or bullied in a bathroom? If so, how did you react and feel? If not, what do you feel has protected you from this kind of behavior?-What are your thoughts about access to restrooms for all people? Do you feel that policies are necessary or a waste of time, effort, and money? Why?-What are some concerns that individuals have about transgender people using bathrooms that are congruent with their gender identity? Do you feel that these are legitimate? Why or why not?” ImportantBe sure to meet or exceed the word requirement and include the word count at the end of your philosophy. The word count should be based solely on your response. If you are using Microsoft Word, you can find the number of words at the bottom of the document and you can also highlight the area in which you want to determine the number of words. Also, please read the grading criteria below so you will know exactly what is expected for this assignment. File UploadGoogle DocGoogle DriveOffice 365Tutor.com 24/7 Homework HelpUpload a file, or choose a file you’ve already uploaded.Upload FileUse Webcam Add Another FileClick here to find a file you’ve already uploadedCancel Submit AssignmentRubricSexual PhilosophySexual PhilosophyCriteriaRatingsPtsThis criterion is linked to a Learning OutcomePart 110 ptsHigh QualityProvided a comprehensive and detailed response that addressed how thoughts and behaviors influence the quality of one’s personal life, relationships, society and the world at large8 ptsModerate QualityProvided a general/basic response about how thoughts and behaviors influence the quality of one’s personal life, relationships, society and the world at large6 ptsLow QualityProvided a vague response about how thoughts and behaviors influence the quality of one’s personal life, relationships, society and the world at large0 ptsIncompleteResponse was incomplete; no submission10 ptsThis criterion is linked to a Learning OutcomePart 210 ptsHigh QualityProvided a comprehensive and detailed response to all prompts8 ptsModerate QualityProvided a general/basic response to all prompts6 ptsLow QualityProvided a vague response to all prompts0 ptsIncompleteResponse was incomplete; no submission10 ptsThis criterion is linked to a Learning OutcomePart 310 ptsHigh QualityProvided a comprehensive and detailed response to all prompts8 ptsModerate QualityProvided a general/basic response to all prompts6 ptsLow QualityProvided a vague response to all prompts0 ptsIncompleteResponse was incomplete; no submission10 ptsThis criterion is linked to a Learning OutcomeWord Requirement5 ptsFull MarksMet or exceeded word requirement3 ptsLow QualityUnder word requirement by five or less words, and/or didn’t include word count0 ptsIncompleteDidn’t meet word requirement by more than five words5 ptsTotal Points: 35
Florida Southwestern State College Mod 5 Gender Stereotypes and Roles Questions
Lxr- α: Molecular Link in Epidermal Microenvironment
Lxr- α: Molecular Link in Epidermal Microenvironment. ABSTARCT The nuclear receptor LXR-α is a transcriptional regulator involved in numerousepidermal processes including proliferation, differentiation, permeability barrierformation, inflammatory responses, skin development and homeostasis. Owing to itscrucial for multiple cell types in the skin, its activation in one skin cell type mayinfluence its expression and activation in other, thereby having a functional impact. Inthis study we investigated the effects that LXR-α activation in keratinocytes would exerton LXR-α expression in melanocytes. For this, we cultured melanocytes from theclinically healthy subjects and them nurtured with the media from the LXR-α activated (by both Ascorbic acid and Atorvastatin along with 22-R hydroxycholestrol) keratinocyte. The DOPA staining verified the growth of melanocytes and the validationfor viability was done by flow cytometry. The results so obtained supported ourspeculation that LXR-α activation in the normal healthy melanocytes may lead to theirapoptosis. Therefore, LXR-α may be a critical player in keratinocyte and melanocytebiology and could be a potential target for skin disease management. INTRODUCTION Epidermal melanocytes form a functional and structural unit with neighboring keratinocyte. There is apparently a close relationship between melanocytes and keratinocytes that is important for melanocyte survival and differentiation. and that may involve keratinocyte-mediated cytokines . Growth factors produced by adjacent keratinocytes regulate the proliferation and differentiation of melanocytes [2-5]. Therefore, changes in keratinocytes function might have a significant effect on melanocyte survival [6, 7]. The LXRs in skin physiology and pathology have evolved rapidly in recent years as they modulate epidermal proliferation, carcinogenesis, differentiation and permeability barrier function, which identifies them as promising drug targets for the treatment of skin diseases. The nuclear receptors LXR-α and LXR-β are expressed in murine and human keratinocytes [8, 9]. LXR activation also stimulates epidermal lipid synthesis, lamellar body secretion and lipid processing in the stratum corneum . LXR-αactivators stimulate keratinocyte differentiation and also promote epidermal permeability barrier homoeostasis . Activation of LXR-αby oxysterols stimulates keratinocyte differentiation, thereby, making LXR-αimportant in keratinocytes differentiation as well [11, 12]. LXR-αis also known to play a key role as metabolic checkpoint that modulates cell proliferation in skin. At proper dosage, synthetic LXR agonists are safe on endothelial cells and may even transrepress inflammatory reactions .It has also been found that LXR-α might be playing an important role in pathogenesis of pigmentary disorders like psoriasis [14, 15]and vitiligo . Changes in the expression of this receptor in various diseased conditions of skin make it a candidate gene worth investigation, as it may be critical players in keratinocyte and melanocyte biology and homeostasis . In this article we characterize the effect of alteration in expression of LXR-α in the keratinocytes influence the survival of the melanocytes. In our previous studies we have already explored the effects of agonists and activators of LXR-α on its own gene expression in keratinocytes. We here report the effect of melanocytes viability following LXR-α activation with Atorvastatin 22R hydroxycholestrol and Ascorbic acid 22R hydroxycholestrolin cultured keratinocytes, with both the cell types derived from of the same the skin biopsy METHODS Selection of the subjects and clinical evaluation This study was approved by the Institutional Ethics Committee. A total number of 6 controls were enrolled, after their informed consent. The age range was 18–40 years. Skin grafts were collected in the phosphate buffer saline (PBS) and immediately transported to the laboratory in ice. Cellular models employed Fresh biopsy specimens were obtained under aseptic conditions in phosphate buffer saline with antibiotics (penicillin and streptomycin). Keratinocyte Cultures: Culturing of keratinocytes derived from skin biopsies of clinically healthy subjects were carried out in Keratinocytes Specific Media containing no antibiotics. The treatment with Atorvastatin 22R hydroxycholestrol and Ascorbic acid 22R hydroxycholestrol was performed. Cells in one of the wells were incubated with 30µM Atorvastatin and the other well was treated with 0.2mg/ml Ascorbic acid or 12 hours . Then 10 µM 22R hydroxycholestrol was added to both the wells and cells were then incubated for 48 hours. Melanocyte Cultures: Culturing of melanocytes derived from skin biopsies of same clinically healthy subjects was carried out in Melanocyte Media Promocell containing no antibiotics. Then the media from the above mentioned treated keratinocytes was transferred to the respective melanocytes cultures for consecutive three days. Cell identification Keratinocytes: To verify that the cells cultured from the skin biopsies exhibited the characteristic signatures of keratinocytes, Melanocytes :DOPA staining To verify that the cells cultured from the skin biopsies exhibited the characteristic signatures of melanocytes, DOPA staining was performed following a modified method previously described . RNA isolation and cDNA synthesis Total RNA was isolated using the Tri Reagent kit (Ambion, Austin, TX, USA), and cDNA was synthesized using the First-Strand cDNA Synthesis kit (Fermentas, St. Leon-Rot, Germany) following the manufacturers’ protocols. Semiquantitative RT-PCR Semiquantitative RT-PCR was used to determine the gene tran- scriptional expression. PCR amplification was performed using the GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA). All primers were synthesized by Sigma (St. Louis, MO, USA). The primer sequences used are given in Table S2. PCR amplification of cDNA was performed in a reaction mixture containing 10X polymerase, 2 ll cDNA template and sterile RNAse-free water added to a total volume of 25 ll. All PCR reagents were from Fermentas. We first amplified a housekeeping gene encoding b-actin, to monitor RNA quality and cDNA synthesis and to ensure that equivalent amounts of cDNA were used in all PCR amplifications. All PCR products were analysed by separation on a 2% agarose gel stained with ethidium bromide. Annexin V staining Cultured melanocytes after culturing in conditioned media were were processed as previously mentioned  before being used for Annexin V staining (Roche, Mannheim, Germany), according to the manufacturer’s instructions. RESULTS Identifications of melanocytes Melanocytes were cultured with conditioned media from treated keratinocytes (both cell types derived from from skin biopsies of the same patient). After getting pure cultures, these cells were characterized by DOPA staining (Figure 1). LXR-α mRNA expression We checked the expression profile of LXR-α gene in melanocytes cultured in the conditioned media was compared to the controls (Figure.2). The aim was to detect any change in gene expression of LXR-α and its effector genes . Results revealed the higher presence of LXR-α mRNA expression in melanocytes cultured in bot the treated conditioned media compared to controls. Effect on the apoptosis Experiments were performed and it was interesting to find that there was an increase in the apoptosis of melanocytes nurtured with the media transferred from the keratinocytes treated Ascorbic acid 22-R hydroxycholestrol i.e. 26% compared to 17.6% in the melanocytes nurtured with the media transferred from the keratinocytes treated Atorvastatin 22-R hydroxycholestrol whereas the control non- treated melanocytes showed 10% apoptotic cell population (Figure 3). DISCUSSION The role multivalent LXR-α has recently been described in many skin diseases. A marked expression of LXR-α has been observed in cells adjacent to dermal papilla, speculating that it may correlate with site of hair melanocytes . Important genes involved in regulation of both keratinocytes and melanocytes are target genes of LXR-α; it can be speculated that LXR-α might be playing the important role in pathogenesis of varied skin disorders and homeostasis . Studies have previously shown that chronic activation of LXR-α in pancreatic β-cell provoked lipid dysregulation and concomitant apoptosis. To verify the speculation, the cultured melanocytes from the clinically healthy subjects were nurtured with the media from the LXR-α activated (by both Vitamin C and Atorvastatin alongwith 22-R hydroxycholestrol) keratinocyte media. The DOPA staining in Figure 1 shows the viable melanocytes which were further validated by FACS and the results so obtained supported our speculation that LXR-α activation in the normal healthy melanocytes may lead to their apoptosis, as LXR-α is known to inhibit cell proliferation and enhance apoptosis (Figure 3). We have already reported that the LXR-α expression was present in human melanocytes and keratinocytes [15, 16]. In this study, we compared the expression of LXR-α in conditioned media from keratinocytes treated with Ascorbic acid 22-R hydroxycholestrol and Atorvastatin 22-R hydroxycholestrol compared to the control and found that mRNA expression of LXR-α was significantly higher in both the treated groups as compared to the control.So, it can be said that there is an LXR-α imbalance in the genesis of skin disorders. Although future studies will reveal whether LXR-α dysregulation in skin cells contributes to the diseased state in vivo, the data presented here suggest a potential target for the development of a successful method of regulating the diseased skin conditions. Not only LXR-α has a robust anti-inflammatory activity in skin, but they also modulate epidermal proliferation, differentiation and permeability barrier function. The abnormal increase in LXR-αexpression in the pancreatic islets of obese and diabetic animal models and the ability of LXR-αligands to induce cell dysfunction suggest the involvement of chronic LXR-αin cell apoptosis  . Keeping in view, the findings reported here coupled with earlier reported findings, it is not unlikely that LXR-α transcriptome may be of crucial importance, not only in understanding of genomic basis of skin disorders it could be useful in designing futuristic therapy for these skin disorders. Lxr- α: Molecular Link in Epidermal Microenvironment