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Endosymbiotic Theory The Modern Or Organelle Containing Eukaryotic Cell Biology Essay

Endosymbiotic Theory The Modern Or Organelle Containing Eukaryotic Cell Biology Essay. In 1883, Andreas Schimper proposed that particular organelles developed from the symbiotic joining of two different organisms, (while he was studying chloroplast division within green plants and observed a similarity between chloroplasts and free living cyanobacteria.), (Vargas-Parada, 2010). In 1905, Konstantin Merezhkovski devised the term, ‘symbiogenesis’ , due to his work on lichens leading him to speculate that “more complex cells evolved from a symbiotic relationship between less complex ones” (Endosymbiosis?, 2006). Then in the 1920s, Ivan Wallin suggested that organelles such as mitochondria and chloroplasts began as symbiotic bacteria and that species creation could have occurred through endosymbiosis. Finally in 1967 Lynn Margulis collected various microbiological observations which supported the hypothesis of endosymbiosis. Evolution of the eukaryotic cell Around 4 billion years ago ancestors of Bacteria and Archaea are thought to have arisen. It is thought that around 3.2 billion years ago, phototrophy occurred, with the common ancestor of many bacteria thought to be an anaerobic phototroph. Then around 2.7 billion years ago oxygen-generating cyanobacteria developed, which over time caused an increase in the atmospheric oxygen levels. The atmosphere slowly changed from anoxic to oxic due to this rise in oxygen levels. At some point during this period eukaryotic microorganisms containing organelles evolved and the oxic environment drove their evolution. Distinguishable features of present day eukaryotes from prokaryotes In many prokaryotic cells, DNA is arranged into a single loop, is circular and unlike eukaryotes, not associated with histones and therefore termed naked. Prokaryotic cells reproduce by the process of binary fission, are mainly unicellular organisms and are usually smaller than eukaryotic cells. In the nucleus of the eukaryotic cell, the DNA is organised into chromosomes, which is surrounded by a membrane. Eukaryotic cells reproduce by the means of mitosis and meiosis, are usually larger than prokaryotic cells and are usually complex and multicellular. The main distinguishing feature of eukaryotic cells is that they contain membrane bound organelles, with the most noteworthy being the mitochondria and chloroplasts. The major organelles involved in the endosymbiotic theory The endosymbiotic theory refers to the organelles mitochondria and plastids, (plastids refer to chloroplasts, chromoplasts, and gerontoplasts, to name a few), but focuses on chloroplasts. Mitochondria Figure : Diagram to show structure of a mitochondrion. are the site of respiration and oxidative phosphorylation in eukaryotic cells. They are membrane bound organelles, with each cell containing thousands of them. Mitochondria show variations in their morphology, and can be rod-shaped or spherical. They are surrounded by an outer membrane and an inner membrane. The outer membrane is made up of protein and lipid and contains channels which allow the diffusion of ions and small organic molecules. The inner membrane is less permeable and is invaginated to form cristae. Oxidative phosphorylation occurs on the inner membrane; with cristae allowing maximum surface area for ATP synthase molecules, which are involved in the synthesis of ATP (Adenosine Triphosphate), the final step of oxidative phosphorylation. It is for this reason that mitochondria are considered as the power generators of the cell. Mitochondria possess their own DNA which is independent of the cell’s chromosomal DNA. The mitochondrial genome mainly encodes proteins needed for oxidative phosphorylation and also encodes ribosomal RNAs, (rRNAs), transfer RNAs, (tRNAs) and proteins necessary for protein synthesis. Mitochondria use simplified genetic codes, which seem to have arisen from selection pressure for smaller genomes (Madigan, 2009 p. 351). Even though mitochondria possess their own genome they still require proteins encoded by nuclear genes. Chloroplasts Figure : Diagram to show structure of chloroplast. are membrane bound organelles found in phototrophic eukaryotic cells. These cells are found in plants algae and some protists. Chloroplasts contain chlorophyll, a photosynthetic pigment, are usually larger than bacterial cells and can differ in size and shape. Chloroplasts again like mitochondria consist of an inner membrane and an outer membrane. Their outer membrane is permeable and the inner membrane is less permeable. The inner membrane surrounds the stroma, which houses the chlorophyll and other cellular components needed for the process of photosynthesis in thylakoids, which are stacked into grana. The stroma of the chloroplast holds large amounts of the enzyme RubisCO, (ribulose bisphosphate carboxylate), which catalyses the Calvin cycle or the dark reactions of photosynthesis. The Calvin cycle converts COâ‚‚ into various organic compounds like glucose which are needed by cells. Chloroplasts also contain their own DNA, which is independent of the cell’s chromosomal DNA. The identified chloroplast genomes are all circular DNA molecules, with each chloroplast containing several matching copies of the genome (Madigan, 2009 p. 350). Genes contained within the chloroplast encode for proteins required for photosynthesis and autotrophy to occur and also rRNA and tRNA used for the processes of transcription and translation. Like mitochondria, chloroplasts have some proteins, which are encoded by nuclear genes and not just by the chloroplast genome. Introduction to the endosymbiotic theory The primary endosymbiotic theory implies that the mitochondrial ancestor was a free living facultatively aerobic alphaproteobacterium, attained by another cell and therefore giving rise to a eukaryotic cell. (Madigan, 2009 p. 520) A species of cyanobacterium is thought to be the ancestor of chloroplasts, obtained by a heterotrophic eukaryote, after eukaryotic cells had appeared around 1.5 billion years ago (Madigan, 2009 p. 520) and was obtained as an internal symbiont (Bruce Alberts, 2002). The diagram below illustrates primary endosymbiosis. Figure : Diagram to illustrate primary endosymbiosis. Secondary endosymbiosis refers to the event where non-phototrophic organisms attained chloroplasts, after the primary endosymbiosis event is said to have occurred. Secondary endosymbiosis is said to have been an important factor in the evolution of eukaryotes, bringing the modern diversity of life into being (Macfadden, 2001). Features of and molecular evidence for the primary endosymbiotic theory The main point of the primary endosymbiotic theory, and which gives it its credibility, was that both mitochondria and chloroplasts both contain DNA, with rRNA, tRNA and proteins involved and needed for the respiratory chain in mitochondria and proteins needed for photosynthesis in chloroplasts, being encoded by these small genomes within mitochondria and chloroplasts. Non-phototrophic eukaryotic cells are genetic chimeras containing DNA from two different sources, the endosymbiont, which is the mitochondria, and the host cell nucleus (Madigan, 2009 p. 520). Eukaryotes which are phototrophic, for example algae and plants, have DNA from two endosymbionts, the mitochondira and the chloroplasts as well as the nuclear DNA. The majority of mitochondrial DNA and chloroplast DNA is similar to bacterial DNA in its shape, which is circular and size. The second feature of primary endosymbiosis is that genes originating from bacteria are found in the nucleus of the eukaryotic cell. This has been proven by sequencing genomes, which have shown that nuclear genes encode properties unique to mitochondria and chloroplasts, (and also which closely resemble genes of bacteria), showing that during the evolution of the eukaryotic cell these genes where transferred to the nucleus of the eukaryotic cell, from the bacterial endosymbionts, during the development of the organelle from the engulfed cell (Madigan, 2009 p. 521). The third feature of primary endosymbiosis is that the mitochondria and chloroplasts contain their own ribosomes. Ribosomes are present in eukaryotic cells and prokaryotic cells, with eukaryotic cells possessing the larger form, 80S, and prokaryotic cells containing the smaller, 70S, ribosomes. The mitochondria and chloroplast contain these 70S ribosomes. The fourth feature is antibiotic specificity. Mitochondria and chloroplast are sensitive to antibiotics which kill bacteria or are bacterial inhibitors. Some for example streptomycin do this by specifically interfering with the functions of the 70S ribosomes, which occurs in the same way in mitochondria and chloroplasts (Madigan, 2009 p. 521). Rifampicin is an antibiotic which in bacteria affects the RNA polymerase. It does not have this effect on eukaryotic RNA polymerase however does inhibit mitochondrial RNA polymerase. The fifth and final feature of primary endosymbiosis is that of molecular phylogeny. Phylogenetic studies comparing organelles and rRNA support the theory of mitochondria and chloroplasts deriving from bacteria. Other evidence includes: Both mitochondria and chloroplast are surrounded by two membranes. It is though that the inner membrane is the original membrane which contained the prokaryotic cell and the outer membrane results from the process of endocytosis when the bacteria were taken into the eukaryotic cell. Protein synthesis in the endosymbionts begins with N- formyl methionine, the same amino acid that initiates protein synthesis in bacteria whilst in eukaryotic cells protein synthesis is initiated by methionine. The thylakoid membrane and the protein complexes which it contains are like those that can be found in cyanobacteria (Pyke p. 5) , and chloroplasts can divide in a way which is similar to the process of binary fission. This evidence suggests that the host cell which obtained the mitochondrion by phagocytosis was an anaerobic eukaryotic cell, (which already contained a nucleus) and that the mitochondrial endosymbiont was an obligate aerobe (Mentel, 2010). This event was later followed by the endosymbiosis of a cyanobacterium, allowing the eukaryotic cell to become photosynthetic. In this way the host eukaryotic cell attained permanent organelles which are suited to energy production. It was also mutually beneficial for the symbionts which obtained a constant and accommodating environment for their growth. Secondary endosymbiosis The primary endosymbiosis event in the common ancestor plants and in green and red algae is said to have created the modern day chloroplast. The secondary symbiotic event refers to non-phototrophic eukaryotes obtaining chloroplasts by the endocytosis of a green or red algal cell and consequently becoming phototrophic. It is thought that secondary and even further endosymbiosies lead to the diversification of eukaryotes. Problems with the endosymbiotic theory To support the endosymbiotic theory in the 1980s, Tom Cavalier-Smith proposed that certain single-celled eukaryotes which are at present still living, bear a resemblance to earlier eukaryotes, were ‘primitively amitochondriate’ (Lane, 2005 p. 42),(basically eukaryotes without any mitochondria), and Cavalier-Smith named them ‘archezoa’. (He later abandoned this theory in 2002, (Cavalier-Smith, 2002)) These cells derived their energy via fermentation and prefer environments with little oxygen and were thought to be the bridge between early eukaryotic cells (as archezoa contain a nucleus) and prokaryotic cells, which would have supported the theory of a bacterial cell being phagocytised, but not digested, leading to the possession of a mitochondria in the early eukaryotic cell. It is thought that as oxygen levels on earth started to rise, the mitochondria too began to evolve, due to aerobic respiration being more resourceful than anaerobic respiration, which again supported that the phagocytosis of this bacterial cell was for the purpose of a more efficient way of generating energy. In March 2000, Jan and Siv Andersson proposed the Ox-Tox hypothesis to explain that aerobic respiration was the selective pressure leading to the gain of the mitochondria, originally selected for the removal of oxygen by the host cell (Andersson, 2000). This was due to research showing that the genome sequence of Rickettsia prowazekii had a strong phylogenetic relationship with mitochondrial proteins (Andersson, 2000). However in the late 1990s these hypotheses were question due to the appearance of contradictive evidence. The genomes of archezoa were sequenced and genes were found which showed that the ancestors of archezoa once had mitochondria, even though there was no physical indication of them in the cell. Some ‘archezoa’ such as Giardia were found to have mitochondria but in the form of mitosomes, which still carried out mitochondrial processes (Lane, 2005 p. 47) again implying that these eukaryotes once contained mitochondria within their cells. It was then suggested that the original host in the eukaryotic merger was a methanogen, due to the structure of eukaryotic and methanogenic histones and the 3D conformation of the DNA associated with the histones being closely related (Lane, 2005 p. 48). Chloroplasts aren’t the main focus when disproving the endosymbiotic theory as it’s thought that when the phagocytosis of a phototrophic symbiont occurred, the host cell already contained mitochondria. The alternative theories to the mainstream endosymbiotic theory The hydrogen hypothesis In 1998, William Martin and Miklós Müller proposed the hydrogen hypothesis. Unlike other hypotheses which suggested that the formation of eukaryotic cell was due to the rising oxygen levels, this hypothesis suggested it was to do with hydrogen. They state that “Eukaryotes are suggested to have arisen through symbiotic association of an anaerobic, strictly hydrogen dependent, strictly autotrophic archaebacterium (the host) with a eubacterium (the symbiont) that was able to respire, but generated molecular hydrogen as a waste product of anaerobic heterotrophic metabolism.” (Müller, 1998). The hydrogen hypothesis is based on hydrogenosomes which are anaerobic mitochondria, that produce ATP and with their waste products including hydrogen gas and carbon dioxide and which also are able to function in low oxygen environments. Martin and Müller suggested that there was a methanogenic ancestry of the host (Müller, 1998) due to methanogens needed the very products (hydrogen and carbon dioxide), which are released by hydrogenosomes and because hydrogenosomes survive in the same environment as methanogens. Due to the intimate association between the methanogen and the bacterium, the symbiosis becomes closer, due to the methanogen needing the products of respiration produced by the bacteria and it is gradually engulfed by the methanogen. By the process of lateral gene transfer from bacterium to methanogen, the methanogen is now able to carry out aerobic and anaerobic respiration (Lane, 2005). There are known examples today of bacteria which live as endosymbionts within other prokaryotes (Mentel, 2010), which again helps support this hypothesis. Another point which gives the hydrogen hypothesis credibility is that all anaerobic and aerobic mitochondria all originated from a facultatively anaerobic ancestral state (Mentel, 2010). Also according to the hydrogen hypothesis, the host cell was not required to have possessed a nucleus (Müller, 1998). The formation of the nucleus is explained by genes for the synthesis of lipids being transferred to the host chromosome from the symbiont leading to the host synthesising these bacterial lipids and then resulting in the formation of the endoplasmic reticulum, an internal membrane system and origination of a eukaryotic nucleus (Madigan, 2009 p. 376). The effects of the hydrogen hypothesis for chloroplasts have not been considered in much depth (Allen, 2003). The syntrophy hypothesis This hypothesis was proposed by Purificación López-García and David Moreira in 1999. This hypothesis is based on similar metabolic considerations as the hydrogen hypothesis proposed by Martin and Müller (Moreira, 1999). There are some similarities between the two hypotheses. Firstly, they both maintain that the metabolic nature if the original symbiotic event involved both hydrogen and syntrophy, secondly that the hydrogen dependent autotrophic archeon must have been a methanogen (Moreira, 1999) and finally that the mosaic nature of the eukaryotic genome can be explained by ‘lateral gene transfer and replacement over a long symbiotic life’ (Moreira, 1999). The difference between these hypotheses is that the syntrophy hypothesis states that the organisms involved were δ- proteobacteria, which are ancestral sulphate reducing myxobacteria, and methanogenic archaea (Moreira, 1999). The hydrogen hypothesis states that an α-proteobacteria began the symbiosis, whereas in the syntrophy proposal it is thought that two eubacterial types were involve, a sulphate reducing δ- proteobacteria, (which produced hydrogen for fermentation), and also an α-proteobacterial methanotroph became symbionts and used the methane which was generated by the methanogen to produce C0â‚‚. Both of these hypotheses agree that the ancestral mitochondria were of anaerobic origin (Moreira, 1999). The chimeric nature of the eukaryotic cell The hypotheses mentioned above, suggest that the modern eukaryotic cell is a chimera, which is a cell that is made up of features from both Bacteria and Archaea (Madigan, 2009 p. 376). Eukaryotes contain the same sort of lipids which are found in Bacteria and the tools they contain for transcription and translation are like those found in Archaea (Madigan, 2009 p. 376). “The chimeric nature of nuclear genomes can be partially explained by the endosymbiont hypothesis of mitochondria” (Hua). At some stage in eukaryogenesis, cellular and genomic combination of the two different organisms (which vary for the hydrogen and syntrophy hypothesis) happened with gene transfer from bacteria to archaea and then subsequent replacement (as horizontal gene transfer, which includes the introduction and loss of genes, is often seen in symbionts (Madigan, 2009 p. 377)). The bacterial genome is then thought to have condensed down and also degraded as the cell underwent evolution, The developing eukaryotic cell must have inherited some of the archaeal-DNA processing systems, whereas the cellular metabolism systems are thought to have come from bacterial organotrophy (Hua). Conclusions During its evolution the modern day eukaryotic cell has become ever more complicated and has led to the appearance of complex, multicellular life forms which are powered by mitochondria and chloroplasts in phototrophic eukaryotic cells. The progression of its evolution and how all the individual compartments of the cell occurred is expected to have taken an extensive amount of time. Variation and natural selection evidently occurred, perfecting the cell by the removal of mechanisms and cellular structures which were deemed useless, leading to biological diversity. It is still undetermined which of these hypotheses mentioned accurately describe the origin of eukaryotes and is thought that the further sequencing of genomes and phylogenetic analyses of the prokaryotic and eukaryotic genomes will provide an improved elucidation of the origin and diversity of eukaryotes. Word Count: 2,996 Endosymbiotic Theory The Modern Or Organelle Containing Eukaryotic Cell Biology Essay
Unit 3 essay.

This assignment provides you with an opportunity to explain the four infrastructures influencing e-commerce strategy.
Begin by selecting an e-commerce out on the Internet, excluding the examples in the textbook. Then, draft a two-page
essay that explains how the e-commerce’s strategy has been influenced by the four infrastructures:

 Layer 1: network technology substrates,

 Layer 2: transport services and representation standards,

 Layer 3: middleware services, and

 Layer 4: applications.
If you are not sure about the strategy that the e-commerce actually used, then you can make logical estimations or
recommendations for how your strategy, for the selected company, would be influenced by the four infrastructures. As
part of your explanation, be sure to include how each layer is dependent upon other layers.
There is no need to prepare a title page nor a references page; however, be sure to use Times New Roman, 12-point font,
double-spacing, and one-inch margins.
Information about accessing the Blackboard Grading Rubric for this assignment is provided below.
Unit 3 essay

Gyn-affection in Literature Essay

There is no precise definition of such phenomenon as gyn-affection. In a few words, it can be defined as a friendship between women but certainly it would be a very simple derfinition. Gyn-affection can mean passion that women feel for each other, this phenomenon can have a bit political context as well. Janice Raymond, a novelist, coined the term to illustrate a relationship, which includes not only fondness and affection, but also creates a sense of mutual empowerment among females (Jolene 2012, P.1). Affection in this sense means the state of influencing, acting upon, moving, impressing, being influenced, being impressed, and acted upon by other women. In her writings, Raymond says that women could liberate themselves only if they embraced a gyn-affective life and rejected most of the lies perpetuated against them. Gyn-affection in “The Color Purple” In her book “The Color Purple,” Alice Walker described how male African Americans in the mid 1900s oppressed their women (Walker 1982, p. 5). In this patriarchal society, most women were illiterate and continuously oppressed by men. Using the female network, the author illustrated that through unity, strength and support, women could rise and ifght against their opressors. Gyn-affection in the book has been portrayed in different forms, particularly in motherly, sisterly and womanly ways. For instance, the author referred to the relationship between Nettie and Celie as motherly (Walker 1982, p. 45). When Nettie’s mother died, Celie took care of Nettlie as if she was her own daughter. For the greater part of her life, Nettie saw love only from her sister and gave back love to her. Get your 100% original paper on any topic done in as little as 3 hours Learn More The sister’s motherly love was portrayed through Celie’s selfless sacrifices for her younger sister. Later on, the letters that Nettie wrote to her sister expressed in many ways her gratitude for Celie’s gift of survival. Without one another, female characters in this novel would be less powerful. After Celie met Shug, a blues singer, she immediately developed a strong friendship with her (Walker 1982, p. 67). Through their intimate relationship, Shug instills self-confidence and self-esteem in her. As a result, the author noted that Celie later on felt more comfortable and respected herself for the first time in her life. In addition, Celie developed very strong love for Shug because she helped her come into terms with herself. Another woman who helped Celie realize the power of her voice was Sofia. Sofia, as Celie’s close friend, would not allow men to assert their dominance over her. Like Shug, Sofia taught Celie that she must stand up for herself or men would oppress her. Afterwards, Celie learned to stand up for herself and eventually achieved happiness and respect. To assert the importance of gyn-affection, the author demonstrated that only women with independent economic position were able to stand up for themselves. As such, she showed that women’s conditions can greatly improve if they unite and support each other. Gyn-affection in other texts Gyn-affection is depicted not only in “The Color Purple” but also in other feminist books. Many women activists have written numerous books dwelling on gyn-affection. We will write a custom Essay on Gyn-affection in Literature specifically for you! Get your first paper with 15% OFF Learn More Among these books are “Handmaid’s Tale” and “A Passion for Friends.” In both novels, the protagonists are women who were oppressed and dominated by men. In both texts, women were affected by similar situations, but eventually managed to become stronger after interacting with other women. In the “Handmaid’s Tale,” the author incorporated many feminist theories and frequently referred to female characters to emphasize the impact of gyn-affection. Offred, the narrator in the story, described her experience as a handmaid in a patriarchal society (Atwood 1986, p.15). Offred with the help of her friend Moira finally became motivated and courageous. Just like in teh book of Alice Walker, women in the “Handmaid’s Tale” are forced to work together to get rid of the male oppressors. Aunt Lydia claims that women in Gilead society could live free from rape, fear, and harm if they could only unite and build a unified female society (Atwood 1986, p. 23). Another book that depicts the effectiveness of gyn-affection is “A Passion for Friends” written by Janice Raymond. In this book, Raymond affirmed the importance of female friendship (Raymond 1986, p. 9). Like Alice Walker, the author illustrated that women could overcome all the obstacles in their way by acting as a team. Raymond saw the possibility of female friendship without boundaries. Unlike other texts, “A Passion for Friends” states that women have been friends for decades and that their relationships have been distorted and destroyed by self-vested men. Raymond still believes that despite enormous pressures put on women by men, women can collectively work together to eradicate these vices (Raymond 1986, p. 11). Not sure if you can write a paper on Gyn-affection in Literature by yourself? We can help you for only $16.05 $11/page Learn More In this regard, Raymond asserted that some women have always existed for women. Thus, she called women to identify such women and work together to attain their self-worth. The three books showed the power of female friendship and various ways how women can survive in oppressive situations and resist men’s opprecise behaviour (Brien 2010, p. 34). In addition, three authors showed that women should form strong units to prevail in male dominated societies (Brown

SOCW 6210 Walden Empirical Research & Developmental Theory Sexual Orientation Research

essay writer free SOCW 6210 Walden Empirical Research & Developmental Theory Sexual Orientation Research.

What does evidence-based research say about the development of sexual orientation in young and middle adulthood? Though the body of literature addressing sexual orientation, including heterosexuality, homosexuality, and bisexuality, is far from complete, the literature continues to grow. Your awareness of this literature helps inform your social work practice as you address the needs of clients with a variety of sexual orientations.For this Assignment, conduct your own research to identify at least one journal article that addresses sexual orientation. Select an article that you find especially relevant to you in your role as a social worker.Submit a 2- to 4-page paper that includes the following:A summary of your findings regarding sexual orientation and its impact on life-span development, including findings from the resources and from the journal article(s) you selected during your researchAn explanation of how you might apply your findings to social work practiceSupport your Assignment with specific references to the resources. Be sure to provide full APA citations for your references.
SOCW 6210 Walden Empirical Research & Developmental Theory Sexual Orientation Research

University of British Columbia Describe the Role of Climate Change on Natural Disasters Discussion

University of British Columbia Describe the Role of Climate Change on Natural Disasters Discussion.

Assignment: Climate Change and Environmental Justice When a disaster occurs, media outlets often focus on the images of the initial impact of the destruction and the grief-stricken faces of survivors. After the rubble begins to be cleared away, however, media outlets typically lose interest and fail to report on the recovery process for the survivors. However, long after the media fades, individuals continue to need help and assistance, whether due to displacement or loss of livelihood. For this Assignment, think about the influence of climate change and effects of natural disasters. Reflect on the disparate impact on diverse communities and the role of social workers in preventing or intervening with impacted communities. The Assignment (2–3 pages): Describe the role of climate change on natural disasters.Explain the concept of environmental justice as it relates to disasters caused by climate changes.Explain the role of social workers to mitigate and ameliorate the effects of climate change.
University of British Columbia Describe the Role of Climate Change on Natural Disasters Discussion

ENG 101 WCU Personal Writing about Miami & Writing a Haiku Writing Worksheet

ENG 101 WCU Personal Writing about Miami & Writing a Haiku Writing Worksheet.

Hello below are the instructions for the assignment. For this assignment, you will create a haiku and an acrostic. The first one will be about your hometown.Steps:Write a short paragraph (3-5 sentences) about your hometown. Indicate its location and some interesting details about it.Write a haiku (3 lines with a 5,3,5 syllables pattern).The second poem is an acrostic. It must be about someone you love or admire.Steps:Write a short paragraph (3-5 sentences) about the person you chose.Write your acrostic.You have to deliver both tasks in a Word file that includes a cover page with your name. The short paragraph should be written in Times New Roman, 12.
ENG 101 WCU Personal Writing about Miami & Writing a Haiku Writing Worksheet

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