I would like to introduce yourself to everyone by explaining 4-5 sentences why a particular work of art appeals to you. (Posted below). In this chapter, we have a very broad definition of art. Basically, anything visual communicates an idea. So this could be a museum thing, an advertisement, a standard cover, architecture, a tattoo, etc. What do you like about the artwork you’ve chosen? why? Submit your answer of four to five sentences. Make sure to include a photo of your artwork in your post. You can do this by using the “Embed Image” button in the toolbar. (It’s a rectangle with two triangular mountains and a circular sun.) You will have to upload an image to your Canvas account first via the ‘Photos’ tab in the top right. You can also easily post a picture by copying it from your source and then pasting it into your discussion entry.
ART 1 Barstow Community College An Appealing Work of Art Discussion
Instructions Please read the article “Where Have All the Parents Gone?” again and take notes on all the main
Instructions Please read the article “Where Have All the Parents Gone?” again and take notes on all the main points the author has made. Then read the attached piece and compare your in class writing on the solutions to bad parenting against the points made in the article. Looking at all the material shared included the PEW research on parenting, write a 1-2 page report on where we are TODAY as a society when it comes to having and raising strong and successful children.
HSA 300 Strayer University Decriminalization of Marijuana Discussion
order essay cheap HSA 300 Strayer University Decriminalization of Marijuana Discussion.
Research where your representative stands on a current health-care issue as well as where the medical profession, such as professional organizations, practicing medical professionals, patients, and other advocates, stands on the issue. In your letter, you will state why you either agree or disagree with your representative’s position on the issue and provide references to support your positionIdentify a health-care issue that is currently being debated nationally or at the state level.Write a two-page letter explaining why you agree with your representative’s position or not.Include data and evidence as appropriate to align your position with the current People goals. a. https://www.cdc.gov/nchs/healthy_people/hp2020/hp2020_topic_areas.htm b. Include references to the appropriate government agencies.
HSA 300 Strayer University Decriminalization of Marijuana Discussion
The Design Industry: Past Experience and New Trends Essay
Table of Contents Introduction The Interview Summary Conclusion Works Cited Introduction Interviewing is one of the important ways to find out the views of people on various issues. I used the method of interview for the purposes of my analysis. I initiated the conversations with the representatives of the design industry in order to determine the major changes, which occurred in design during the past decades. The Interview Summary Last week, I was lucky to talk to the representative of the design industry. I think that the results of my work can be used to summarize the major changes occurred in the field of design. The conversation with the designer allowed me to get a better understanding of the peculiarities of their work. In particular, in the interview with Amanda Johns, an interior designer, working for one of the successful firms in the city, I got to know that the design industry underwent the substantial changes during the last years. She said, “The work of the designer became much more diverse than it was in the past.” When I asked her what she meant under the “diverse”, she said that the modern designers had to be erudite in many spheres and had to be flexible in order to react to the rapid changes, occurring in the modern world, quickly. Frankly speaking, I was wondered when Amanda said that she did not have a designer diploma when he joined the firm. She said that she graduated from university ten years ago and could not imagine that one day she would dedicate her life to the creative activity. At the university, Amanda majored in Math. However, the knowledge, which she obtained, helped her in the designer work. She explained it as follows, “The pencil and paper are no longer the primary mediums for the designer’s drawings. It is the specialized and often rather complicated software, which we use for the creation of images that takes the central place in our working process”. She said that she applied her knowledge of Math to her work as she had to logically think about the parameters of the models, which she created. “I had to reflect the actual proportions of premises in the computer images with the mathematical exactness”, she added. Then, I suggested talking about the current situation in the US designer industry. I said that, according to the statistical data, the Creative Class workers account for approximately 35% of the employed in the US (Florida 268). I asked her to comment on this data. She said, “I think this data is underestimated as a lot of people work in the industry as the freelancers while having a full-time job somewhere else.” The interview raises the questions closely connected with the problems touched upon in the book by Richard Florida. In particular, it is interesting to compare the average cost of education with the average salaries of the Creative Class workers in order to determine how many years it will take to be repaid for the costs incurred. Furthermore, the obtained results can be compared with similar data from other industries. Conclusion In summary, the interview allowed me to gather important information about the design industry, its peculiarities, and trends. It also gave me an indication to the areas of further research. And last but not least, I was spending my time with pleasure while communicating with an interesting person. Works Cited Florida, Richard L. The Rise of the Creative Class: Revisited, New York: Basic Books, 2012. Print. Get your 100% original paper on any topic done in as little as 3 hours Learn More
Gene Mapping Using Markers of Bactrocera Tryoni Report
Table of Contents Introduction Methods Results Discussion Conclusion References Supplementary Information Introduction Bacterocera tryoni, a serious pest of diverse fruits and vegetables, belongs to the family of Tephritidae and is native to Australia, particularly the state of Queensland. It is an appropriate model insect that the study used to illustrate genetic linkage and mapping of visible and molecular markers of the white gene. According to Gilchrist et al. (2014), tephritid fruit flies live in colonies that permit sympatric speciation and the maintenance of pure strains. Moreover, B. tryoni flies are easy to manipulate to get desired hybrids because they have a flexible mating pattern that allows male back cross without the interference of crossing over during meiosis. Comparatively, B. tryoni has white recessive marks on its thorax, which are homologous to white eyes of Drosophila melanogaster. The mapping of the white gene on different chromosomes and microsatellite loci suggests the existence of a linkage to the visible white marks. Gene mapping is essential to create a genetic linkage between the visible white marks and white gene in B. tryoni. In the experiment, male back cross enabled the identification and differentiation of the nature of linkage that exists between the white marks and the white gene. The presence of simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) in the white gene permits RFLP to generate fragments of different lengths. The targeted region of the white gene comprises of 680bp with conserved restriction site (GTAC) and polymorphic restriction site (GTWC) for Rsal. When digested with Rsal, the conserved restriction site in one allele (b) generates two fragments (550bp and 130bp), whereas both conserved and polymorphic restriction sites give three fragments (360bp, 190bp, and 130bp) in another allele (a). RFLP is a molecular marker that effectively differentiates the two alleles of the white gene and enhances the creation of the genetic linkage of white marks. Hence, the purpose of the laboratory report is to describe how amplification of the white gene, restriction fragment lengths, and association analysis lead to the formation of the genetic map that links it to white marks. Methods The parental cross was conducted between two stocks of flies to get the first generation progeny (F1). For white marks, male parent flies in one stock were homozygous wild-types with conserved and polymorphic restriction sites for Rsal (wm /wm Ra /Ra), while female flies in another stock were homozygous mutants with a conserved restriction site for Rsal (wm/wm Rb/Rb). Subsequently, the male back cross was done and genotypes and phenotypes of the second generation progeny (G2) were recorded. The ratios of genotypes and phenotypes were determined and compared to expected ones in linked and unlinked genes. Flies were sampled from parental, the first progeny, and the second progeny and their genomic DNA were isolated. Each demonstration group was assigned 20 samples of genomic DNA constituting of the male parent (MP), female parent (FP), first generation (F1), 16 second generations (G2), and negative control. The polymerase chain reaction (PCR) was done using a thermocycler to amplify the target region of the white gene in the isolated DNA samples of respective flies. The PCR master mix was prepared with the final concentration of 16.6mM [NH]SO4, 0.5mM dNTPs, 67mM Tris-HCl (pH 8), 3mM MgCl2, 0.45% Triton X-100, 0.2mg/ml gelatin, 12.5μM primer mix, and 0.35units/μl Taq polymerase. The amplification was done on a 25μl reaction volume comprising of 18μl, 2μl, 3μl, and 2μl of the master mix, primer mix, genomic DNA, and Taq polymerase, respectively. The PCR reaction contents were mixed thoroughly and placed in a thermocycler for amplification. The thermocycler was set and the amplification process was allowed to run for about three hours. The PCR conditions were initial denaturation for 3 minutes at 94°C and final extension for 5 minutes at 72°C with cycling times of denaturation for 1 minute at 94°C, annealing for 1 minute at 60°C, and extension for 1 minute at 94°C. After completion of the amplification process, amplicons were removed and stored in a freezer at -20°C to preserve them for the next procedure. The PCR product volume of 25μl for each sample was divided into two equal portions for genotypic analysis. One portion of the PCR product was digested with RSal for RFLP analysis, whereas the other portion was not digested. PCR products (12.5μl), 1.75 units/µl Rsal, sterile MQ water, and 10X restriction buffer with 100mM MgCl2, 500nM NaCl, 100mM Tris-HCl, and 100mM DTT were used to carry out restriction digest. The mixture of restriction digest (20μl) was prepared by adding 10μl of PCR product, 6μl of MQ water, 2μl of 10X buffer, and 2μl Rsal into a 1.5 ml sterile microfuge tubes for each sample. The digestion mixtures for all samples were incubated in a water bath set at 37°C for 1 hour. Agarose gel electrophoresis was utilised to analyse DNA fragments obtained from the amplification of the white gene and digestion by Rsal. Gel electrophoresis apparatus constituting of gel tanks, video camera, power pack, UV filter, and UV transilluminator was set up in the laboratory. Moreover, 2% agarose, 1X sodium borate buffer, 25ng/μl pUC19/Hpall standard, and 25ng/μl 1kb ladder were prepared for electrophoresis. The DNA analysis was done by loading 20μl of digested PCR fragments, 12μl of undigested PCR fragments, positive control samples, and genomic standards, as illustrated in Tables 4 and 5 (Supplementary Section). The voltage of electrophoresis was set at 180 and allowed to run until the dye front reaches a considerable distance to allow separation of fragments. Ultimately, the gels were read using UV transilluminator and images were taken for the analysis, as indicated by Figures 1 and 2 (Supplementary Section). Get your 100% original paper on any topic done in as little as 3 hours Learn More Results The estimated fragment sizes of undigested and digested amplicons were tabulated in Table 1 and Table 2, correspondingly. Comparison of the estimated and the expected band sizes indicates that the undigested fragments did not deviate significantly from 680bp. The estimated fragment sizes of the undigested amplicons ranged from about 600bp to 750bp. Table 1. The estimated fragment sizes of undigested amplicons. Samples Estimated Fragment Size (bp) Samples Estimated Fragment Size (bp) Male Parent 680 blank Female Parent 600 blank F1 1000 blank G2 S1 G2 S10 680 G2 S2 600 G2 S11 750 G2 S3 600 G2 S12 680 G2 S4 600 G2 S13 700 G2 S5 680 G2 S14 680 G2 S6 680 G2 S15 700 G2 S7 750 G2 S16 680 G2 S8 680 blank G2 S9 680 No data control 700 blank blank Table 2 shows that the estimated fragment sizes of digested amplicons were 550bp, 360bp, 190bp, and 130bp for heterozygous (RaRb), 550bp and 130bp or 360bp, 190bp, and 130bp for homozygous (RaRa or RbRb). These fragments are of the expected sizes based on restriction digest by Rsal. Table 2. The estimated fragment sizes of digested amplicons. Samples Estimated Fragment Sizes (bp) Samples Estimated Fragment Sizes (bp) pUC19/Hpall 1 kb ladder Male Parent 360, 190, 130 Male control 360, 190, 130 Female Parent 550, 130 Female control 550, 130 F1 550, 360, 190, 130 F1 control 550, 360, 190, 130 G2 S1 550, 360, 190, 130 G2 S10 550, 130 G2 S2 550, 360, 190, 130 G2 S11 550, 360, 190, 130 G2 S3 550, 130 G2 S12 550, 130 G2 S4 550, 130 G2 S13 550, 130 G2 S5 550, 360, 190, 130 G2 S14 550, 360, 190, 130 G2 S6 550, 360, 190, 130 G2 S15 550, 360, 190, 130 G2 S7 550, 130 G2 S16 550, 130 G2 S8 550, 360, 190, 130 No data control G2 S9 550, 360, 190, 130 pUC19/Hpall 1 kb ladder blank The comparative tabulation (Table 3) depicts that phenotypes of white marks link to microsatellites loci of the white gene, Bt1 and Bt7 in chromosome 2, and Bt2 in chromosome 5. While white marks link to homozygous short alleles (SS) in the Bt1 and Bt7 loci, wild-type marks associate with homozygous long alleles (LL) in the same loci. In the Bt2 locus, homozygous long alleles (LL) link to homozygous white RFLP (RaRa), whereas homozygous short alleles (SS) link to homozygous white RFLP (RbRb) and heterozygous alleles (SL) in both Bt1 and Bt2 loci link heterogeneous white RFLP (RaRb). Table 3. Phenotypes for white marks in the identified microsatellites and chromosomes. Fly Sex wm Bt1 Chr2 Bt2 Chr5 Bt5 Chr3 Bt7 Chr2 Bt11 Chr6 Bt15 Chr6 Bt17 Chr4 White RFLP Parent Male WT LL LL SS LL SS LL LL RaRa Parent Female Wm SS SS LL SS LL SS SS RbRb F1 Male WT SL SL SL SL SL SL SL RaRb G2-1 F WM SS SL SL SS LL SS SL RaRb G2-2 F WM SS SL LL SS SL SL SS RaRb G2-3 F WM SS SS SL SS SL SL SL RbRb G2-4 F WM SS SS LL SS SL SS SS G2-5 F WM SS SL SL SS SL SL SL RaRb G2-6 M WM SS SL SL SS SS SS SS RaRb G2-7 M WM SS SS SL SS SS SS SL RbRb G2-8 M WM SS SL SL SS SS SS SS RaRb G2-9 M WT SL SL SL SL SL SL SS RaRb G2-10 F WT SL SS LL SL SS SS SL RaRa G2-11 F WT SL SL SL SL SS SS SS RaRb G2-12 F WT SL SS SL SL SL SL SL RaRa G2-13 M WT SL SS SL SL SL SL SS RaRa G2-14 M WT SL SL SL SL SS SS SS G2-15 M WT SL SL LL SL SL SL SS RaRb G2-16 M WT SL SS LL SL SS SS SL RaRa Discussion Genotypic ratios generated from the banding patterns of the digested and undigested amplicons showed that white marks of B. tryoni link to the white gene. Furthermore, comparison of the distribution of phenotypes and genotypes of white marks revealed that they link to Bt1and Bt7 loci of microsatellites in chromosome 2 and Bt5 locus in chromosome 2. Choo et al. (2017) established that the white gene comprises of seven exons that cover different chromosomes and have genetic linkages with white marks. The existence of numerous microsatellites in B. tryoni complicates mapping of the sequence of genes in the two identified chromosomes. In this view, an accurate mapping of the white gene and white marks requires the use of gene knockout and sequencing of target genes (Choo et al., 2017; Costain, Kannu